Rabbit anti-Human EGFR mAb for FC - P00533

Core Product Specifications and Parameters

Biological Background: Epidermal Growth Factor Receptor Function and Localization

• EGFR is a receptor tyrosine kinase that binds EGF family ligands, triggering homo/heterodimerization, autophosphorylation, and activation of multiple downstream cascades including RAS-RAF-MEK-ERK, PI3K-AKT, PLCγ-PKC, and STAT modules; it also modulates NF-κB signaling and phosphorylates substrates like RGS16 and MUC1 to regulate cell migration and other processes. Related references: PMID:10805725 PMID:27153536 PMID:2790960 PMID:12297049 PMID:15611079 PMID:17909029 PMID:11116146 PMID:11602604 PMID:11483589 PMID:20462955
• EGFR functions as a receptor for hepatitis C virus (HCV), facilitating viral entry into hepatocytes by promoting CD81-CLDN1 receptor complex formation.
• Isoform 2 may act as an antagonist of EGF action, potentially modulating ligand-dependent signaling.
• Subcellularly, EGFR localizes to the cell membrane, endoplasmic reticulum membrane, Golgi membrane, nucleus membrane, endosome, and can be secreted; minor pools are found in the nucleus.
• EGFR is ubiquitously expressed across tissues, with isoform 2 also detected in ovarian cancers.
• Key post-translational modifications and features include phosphorylation, glycosylation, ubiquitination, and palmitoylation, as highlighted by UniProt keywords: host-virus interaction, proto-oncogene, tyrosine-protein kinase, membrane receptor, transmembrane, and signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular ligand-binding domain (aa 335–525) of human EGFR; the resulting rabbit monoclonal antibody likely recognizes native cell surface EGFR. For flow cytometry, perform antibody titration and include isotype controls to optimize staining conditions.
• This conjugate is suitable for live-cell surface staining; if intracellular detection is needed, validate appropriate fixation/permeabilization protocols for your cell type.
• Given EGFR’s broad tissue expression, confirm antibody specificity and signal-to-noise ratio in the desired biological sample (e.g., cancer cell lines, primary hepatocytes) under your experimental settings.
• For HCV entry studies, consider using the antibody to monitor EGFR expression on hepatocyte-derived lines (e.g., Huh7) and evaluating its role in CD81-CLDN1 complex formation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD-grade antibodies and assay-critical raw materials for EGFR-targeted diagnostic and research applications. From validated monoclonal antibodies for flow cytometry to optimized antibody pairs and bulk ancillary reagents, our one-stop sourcing platform supports every stage of diagnostic development. Partner with CamelBio for streamlined procurement, technical consultation, and scalable supply of EGFR-related raw materials tailored to your oncology or infectious disease IVD projects.