Rabbit anti-Human EGFR mAb for FC - P00533

Core Product Specifications and Parameters

Biological Background: EGFR Function and Localization

• EGFR is a receptor tyrosine kinase that binds various EGF family ligands (EGF, TGFA, AREG, etc.) to mediate extracellular-to-intracellular signaling. Related references: PMID:10805725 PMID:27153536 PMID:2790960
• Upon ligand binding, receptor dimerization and autophosphorylation recruit adapter proteins like GRB2, activating multiple downstream cascades including RAS-RAF-MEK-ERK, PI3K-AKT, PLCgamma-PKC, STATs, and NF-kappa-B. Related references: PMID:27153536 PMID:11116146
• EGFR directly phosphorylates substrates such as RGS16 and MUC1, and interacts with CCDC88A/GIV to promote cell migration. Related references: PMID:11602604 PMID:11483589 PMID:20462955
• Isoform 2 may act as an antagonist of EGF action.
• Localized at the cell membrane, endoplasmic reticulum, Golgi, nucleus, and endosomes, with a secreted form also detected.
• Ubiquitously expressed; isoform 2 is also found in ovarian cancers.
• Serves as a host cell receptor for hepatitis C virus (HCV) entry, facilitating CD81-CLDN1 complex formation.
• Post-translational modifications include phosphorylation, glycosylation, palmitoylation, and ubiquitination; member of the protein kinase superfamily.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 335–525 within the extracellular domain of human EGFR, supporting its use for detecting cell surface EGFR by flow cytometry.
• For flow cytometry, optimize antibody titration and include appropriate isotype and Fc blocking controls to ensure specificity.
• Confirm target expression in the relevant cell type; EGFR is ubiquitously expressed but levels vary between tissues and cell lines.
• The reported protein weight (44/69/77/134 kDa) reflects glycosylation and isoform diversity; if adapting for western blot, anticipate multiple bands.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a comprehensive range of IVD raw materials, including validated monoclonal antibodies like this anti-EGFR mAb for oncology and cell signaling research. Our one-stop platform streamlines procurement of bulk ancillary reagents, optimized antibody pairs, and rare target raw materials, supporting development from concept to clinic with reliable supply and technical consultation.