Rabbit anti-Human E-Cadherin/CD324 Monoclonal Antibody for Flow Cytometry - P12830

Core Product Specifications and Parameters

Biological Background: Cadherin-1 (E-Cadherin) Function and Localization

• Cadherin-1 (E-cadherin, CD324) is a calcium-dependent adhesion protein that mediates homophilic cell-cell interactions, critical for maintaining epithelial tissue integrity, cell sorting, and regulating mobility and proliferation of epithelial cells.
  Related references: PMID:11976333
• Plays a role in the early stages of desmosome assembly by facilitating the recruitment of desmoglein-2 (DSG2) and desmoplakin (DSP) to desmosome plaques.
  Related references: PMID:29999492
• Exhibits potent tumor suppressor activity and serves as a ligand for integrin alpha-E/beta-7, contributing to immune cell adhesion.
• E-Cad/CTF2 processing product promotes non-amyloidogenic degradation of amyloid-beta precursor protein, suggesting a neuroprotective role.
• Subcellularly localized to adherens junctions, desmosomes, cell membrane, endosomes, Golgi, trans-Golgi network, cytoplasm, and can be secreted.
• Highly expressed in epithelial tissues including skin, esophagus, and liver, as well as in granuloma macrophages.
  Related references: PMID:27760340, PMID:22294297, PMID:24220297, PMID:3263290
• Endogenous processing yields isoforms at ~90 kDa and ~97 kDa; post-translational modifications include phosphorylation, glycosylation, and ubiquitination.

Experimental Guidance and Technical Tips

• This antibody is validated for flow cytometry; titrate to determine the optimal concentration for your specific cell type and experimental setup.
• For FC, prepare single-cell suspensions and include appropriate isotype controls to ensure specificity.
• The immunogen covers amino acids 155–707 of the extracellular domain, suggesting recognition of an extracellular epitope suitable for surface staining of live, unfixed cells.
• E-cadherin expression is typically high in epithelial cells; loss of expression is a hallmark of epithelial-to-mesenchymal transition (EMT), making this antibody useful for EMT-related studies.
• Although validated for FC, the antibody may be tested in other applications following appropriate validation; in western blotting, expect bands around 90–97 kDa due to processing isoforms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing solution for IVD raw materials, supporting every stage from concept to clinic. For your E-cadherin/CD324 research, we offer validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to accelerate your assay development. Our expertise includes rapid access to rare-target raw materials and tailored technical support, making us a trusted partner for diagnostic manufacturers and research laboratories worldwide.