Rabbit anti-Human E-Cadherin/CD324 mAb for FC - P12830

Core Product Specifications and Parameters

Biological Background: E-Cadherin/CD324 (Cadherin-1) Function and Localization

• Cadherin-1 (E-Cadherin, CD324, encoded by CDH1 gene) is a calcium-dependent cell adhesion protein that mediates homophilic cell-cell interactions, crucial for sorting heterogeneous cell types and maintaining epithelial integrity. Related references: PMID:11976333
• It plays a role in the early stages of desmosome formation by facilitating recruitment of DSG2 and DSP to desmosome plaques. Related references: PMID:29999492
• Acts as an invasive suppressor and is a ligand for integrin alpha-E/beta-7, contributing to the regulation of cell mobility and proliferation.
• Through interaction with AMOTL2, it promotes organization of radial actin fibers and cellular response to contractile forces.
• The cleaved intracellular domain (E-Cad/CTF2) promotes non-amyloidogenic degradation of Abeta precursors, inhibiting APP C99 and C83 production.
• Serves as a receptor for Listeria monocytogenes internalin A, facilitating bacterial uptake.
• Subcellularly localizes to adherens junctions, desmosomes, cell membrane, endosomes, Golgi apparatus, and can be secreted.
• Tissue‑specific expression includes granuloma macrophages, skin, basal esophageal epithelium, and liver, as evidenced at protein level. Related references: PMID:27760340, PMID:22294297, PMID:24220297, PMID:3263290

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 155-707), making it likely accessible for flow cytometry (FC) detection on the surface of live, non-permeabilized cells. Consider titrating the antibody to determine the optimal staining concentration.
• For FC, validate in a relevant human epithelial cell line known to express E-Cadherin (e.g., MCF-7, A-431) and use an appropriate isotype control to confirm specificity.
• The predicted molecular weight ranges from 90 to 97 kDa, potentially reflecting precursor and mature forms. If Western blot confirmation is desired, select molecular weight markers accordingly.
• Note that the antibody is supplied conjugated to ; ensure your flow cytometer is equipped with a suitable laser/filter set (e.g., blue laser 488 nm, green emission).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal antibodies like this E-Cadherin clone, and bulk ancillary reagents to support your cell adhesion and epithelial biology studies. Let us help you source rare-target raw materials and navigate your IVD development journey efficiently.