Rabbit anti-Human DNAJB1/HSP40 Monoclonal Antibody for Flow Cytometry - P25685

Core Product Specifications and Parameters

Biological Background: DNAJB1/HSP40 Function and Localization

• DNAJB1, also known as Hsp40, HDJ1, or HSPF1, is a member of the DnaJ/Hsp40 family of co-chaperones.
• It stimulates the ATPase activity of HSP70 and facilitates the folding of unfolded proteins. Related references: PMID:14503850, PMID:24318877
• Together with TTC1, DNAJB1 acts as a chaperone adapter that regulates HSP70-dependent protein folding. Related references: PMID:14503850
• It negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase of the heat shock response. Related references: PMID:9499401
• Subcellularly, DNAJB1 localizes to the cytoplasm, nucleus, and nucleolus.
• The protein undergoes alternative splicing and phosphorylation, as identified by proteomics.
• DNAJB1 plays a critical role in the cellular stress response and protein quality control.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 241-340) is conserved across both isoforms; verify detection of expected bands at 27 kDa and 38 kDa in relevant cell lysates.
• For intracellular flow cytometry, optimize fixation and permeabilization conditions to ensure antibody accessibility.
• Use an appropriate isotype control and titrate the antibody to determine the optimal staining concentration.
• Validate specificity by knockdown or knockout experiments in the cell system of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For research on protein homeostasis, stress response, and chaperone biology, we can source rare-target raw materials like this DNAJB1/HSP40 antibody to accelerate your assay development and biomarker discovery.