Rabbit anti-Human DNAJB1/HSP40 Monoclonal Antibody for FC - P25685

Core Product Specifications and Parameters

Biological Background: DNAJB1/HSP40 Function and Localization

• DNAJB1, also known as HSP40 or hDj-1, is a co-chaperone that interacts with HSP70 and stimulates its ATPase activity, facilitating the folding of unfolded proteins.
• It may act as a chaperone adapter with TTC1 to regulate HSP70-dependent folding processes.
  Related references: PMID:14503850
• DNAJB1 stimulates the association between HSC70 and HIP, potentially enhancing chaperone complex formation.
• It negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phases of the heat shock response.
  Related references: PMID:9499401
• In vitro, DNAJB1 stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B.
  Related references: PMID:24318877
• DNAJB1 localizes to the cytoplasm, nucleus, and nucleolus, and is involved in stress response. It is subject to phosphorylation and alternative splicing, which may produce isoforms of varying sizes, consistent with the observed 27 kDa and 38 kDa bands.

Experimental Guidance and Technical Tips

• This antibody is designed for intracellular flow cytometry (FC). Ensure fixation and permeabilization steps are optimized for detection of DNAJB1, which may be located in the nucleus or cytoplasm.
• The immunogen sequence corresponds to a region within amino acids 241-340 of human DNAJB1; cross-reactivity with other species has not been tested. Validate in the relevant sample system if using non-human samples.
• Protein weight may vary due to alternative splicing; bands around 27 kDa and 38 kDa may be observed in Western blot applications if validated independently.
• Heat shock treatment of cells may upregulate DNAJB1 expression, potentially improving detection sensitivity.
• For multiplex experiments, confirm that the fluorophore does not interfere with other channels in your cytometry panel.

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CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to support diagnostic development. With expertise in stress biology and chaperone targets, we can assist in sourcing rare or custom reagents to accelerate your research and product pipelines. Explore our comprehensive catalog to find the right materials for your assay needs.