Rabbit anti-Human DLL4 Monoclonal Antibody for Flow Cytometry - Q9NR61

Core Product Specifications and Parameters

Biological Background: Delta-like protein 4 (DLL4) Function and Localization

• DLL4, also known as Delta-like protein 4 or Delta4, is a transmembrane ligand for Notch receptors that activates NOTCH1 and NOTCH4, initiating the Notch signaling cascade (PubMed:11134954). Related references: PMID:11134954
• As a key regulator of angiogenesis, DLL4 negatively controls endothelial cell proliferation, migration, and angiogenic sprouting, ensuring proper blood vessel patterning (PubMed:20616313). Related references: PMID:20616313
• During spinal cord neurogenesis, DLL4 restricts V2a interneuron specification, contributing to neuronal diversity (PubMed:17728344). Related references: PMID:17728344
• In the retina, DLL4 is required for progenitor proliferation and suppression of rod photoreceptor cell fate, facilitating generation of other retinal cell types.
• DLL4 is predominantly expressed on the cell membrane of vascular endothelial cells, consistent with its role in juxtacrine signaling.
• The protein structure features multiple EGF-like repeats, a transmembrane helix, and a cytoplasmic tail, with glycosylation and disulfide bonds supporting stability and ligand-receptor interactions.
• Genetic variations in DLL4 have been associated with developmental disorders, highlighting its critical role in human health.

Experimental Guidance and Technical Tips

• As an -conjugated antibody, this reagent is ready for direct flow cytometry without secondary antibody staining. Consider titrating the antibody to determine the optimal concentration for your cell model.
• The immunogen spans the extracellular domain (amino acids 27–524), which contains Notch interaction regions, suggesting the antibody likely recognizes a functional epitope. Validate sensitivity and specificity in your target cell system.
• Given high DLL4 expression in vascular endothelium, consider using endothelial cell lines or primary endothelial cells as positive controls. For negative controls, include cells with low or absent DLL4 expression.
• For reliable flow cytometry results, incorporate live/dead cell discrimination, isotype controls, and fluorescence-minus-one (FMO) controls to set accurate gates.
• If signal is weak, verify that the antigen is properly expressed on the cell surface; DLL4 may be internalized upon ligand binding, so consider incubation conditions.

CamelBio: Your One-Stop Sourcing Bridge

This anti-DLL4 monoclonal antibody supports research into Notch-driven angiogenesis and neurodevelopment, and CamelBio is your comprehensive partner for IVD raw material procurement. From validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare target sourcing, we provide diagnostic manufacturers, labs, and research institutes with one-stop access to high-quality raw materials, technical services, and consulting—from concept to clinic. Contact us to explore how we can supply the critical reagents that accelerate your diagnostic development.