Rabbit anti-Human Desmoglein 3/PVA Monoclonal Antibody for Flow Cytometry - P32926

Core Product Specifications and Parameters

Biological Background: Desmoglein-3 Function and Localization

• Desmoglein-3 (DSG3), also known as 130 kDa pemphigus vulgaris antigen (PVA) or cadherin family member 6 (CDHF6), is a calcium-binding transmembrane glycoprotein and a member of the desmoglein family.
• A core component of desmosome cell-cell junctions, DSG3 positively regulates cellular adhesion and is necessary for adherens and desmosome junction assembly in keratinocytes under mechanical force.
  Related references: PMID:31835537
• Essential for anchoring telogen hairs: mediates cell-cell adhesion between cells surrounding the telogen hair club and the basal outer root sheath epithelium.
  Related references: PMID:9701552
• Required for epithelial barrier integrity by promoting desmosome-mediated intercellular attachment of suprabasal epithelium to basal cells (By similarity).
• Subcellularly localized to the cell membrane, desmosomes, tight junctions, and cytoplasm, reflecting its dynamic roles in adhesion and signaling.
• Highly expressed in the skin and mucosa, including the basal/spinous epidermis, esophageal mucosa, and hair follicle outer root sheath.
  Related references: PMID:19717567, PMID:22294297, PMID:30528827, PMID:24220297
• Participates in mechanotransduction by forming a complex with PKP1 and YWHAG to localize YAP1 at the plasma membrane, potentially influencing cell proliferation.
  Related references: PMID:31835537
• Inhibits keratinocyte migration via suppression of p38MAPK signaling, suggesting a role in moderating wound healing.
  Related references: PMID:26763450

Experimental Guidance and Technical Tips

• This -conjugated antibody is optimized for direct flow cytometry; determine the optimal working dilution by titrating on a relevant positive cell line (e.g., human keratinocytes or DSG3-expressing cells).
• Ensure proper controls: use an isotype control conjugate and include unstained cells; consider a blocking step with Fc receptor blocker if using cells with high Fc receptor expression.
• The immunogen covers the extracellular region (aa 50–615); therefore, the antibody likely binds an extracellular epitope—avoid permeabilization unless intracellular staining is desired, as it may alter binding.
• For multi-color flow cytometry panels, validate spectral compatibility with other fluorophores and confirm no cross-reactivity with other desmoglein family members in your experimental system.
• If adapting this antibody for other applications (e.g., immunofluorescence or Western blot), verify performance under appropriate conditions and confirm the observed band at ~108 kDa in the relevant sample lysate.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. This desmoglein-3 antibody supports studies in autoimmune blistering diseases and epithelial cell biology. For your next project, rely on CamelBio’s integrated supply chain to deliver high-quality reagents from discovery through clinical application.