Rabbit anti-Human Cytokeratin 8 Monoclonal Antibody () for Intracellular Flow Cytometry - P05787

Core Product Specifications and Parameters

Biological Background: Cytokeratin 8 Function and Localization

• KRT8 encodes keratin, type II cytoskeletal 8, a structural component of intermediate filaments in simple epithelial cells.
• Together with KRT18, it forms the keratin intermediate filament network, providing mechanical stability and resilience to epithelial cells.
  Related references:
  PMID:26823019
• It also protects cells from non-mechanical stress, such as apoptosis (By similarity).
• Required for ARHGEF40-mediated actin stress fiber formation and tensional force-induced stress fiber reinforcement.
  Related references:
  PMID:26823019
• In striated muscle, together with KRT19, may contribute to linking the contractile apparatus to dystrophin at costameres.
  Related references:
  PMID:16000376
• Subcellular localization includes cytoplasm, nucleoplasm, and nucleus matrix, highlighting its roles in both cytoskeletal and nuclear organization.
• Tissue specificity: observed in muscle fibers at costameres, and expressed in gingival mucosa and hard palate.
• Post-translational modifications such as acetylation, phosphorylation, methylation, and ubiquitination regulate its filament dynamics and interactions.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human Cytokeratin 8 (amino acids 1-483), which may support recognition of native protein conformations in intracellular flow cytometry.
• For intracellular staining, optimize permeabilization and fixation protocols to ensure antibody access to cytoplasmic and nuclear compartments.
• Cross-reactivity has been validated for human; when using other species, consider sequence homology checks and pilot validation.
• dye typically has excitation/emission maxima around 590/617 nm, making it suitable for standard flow cytometry detection channels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories one-stop access to IVD raw materials, technical services, and consulting. This anti-Cytokeratin 8 monoclonal antibody supports research into epithelial cell biology, cytoskeleton dynamics, and related disease mechanisms. Our supply capabilities include validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your development from concept to clinic.