Rabbit anti-Human Cytokeratin 8 Monoclonal Antibody for Intracellular Flow Cytometry - P05787

Core Product Specifications and Parameters

Biological Background: Cytokeratin 8 Function and Localization

• Keratin 8 (K8/CK8), encoded by the KRT8 gene, is a type II keratin intermediate filament protein that forms obligate heteropolymers with its type I partner KRT18 in simple epithelial cells (PubMed:26823019).
  Related references: PMID:26823019
• KRT8 provides mechanical stability and resilience to epithelia and protects cells from non-mechanical stress, such as apoptosis (By similarity).
• It is required for ARHGEF40-mediated actin stress fiber formation and tensional force-induced stress fiber reinforcement (PubMed:26823019).
  Related references: PMID:26823019
• In striated muscle, KRT8 and KRT19 may link the contractile apparatus to dystrophin at the costameres (PubMed:16000376).
  Related references: PMID:16000376
• Subcellular localization is predominantly in the cytoplasmic cytoskeleton, but it is also detected in the nucleoplasm and nuclear matrix.
• Post-translational modifications include acetylation, phosphorylation, methylation, ubiquitination, and glycosylation, which modulate its assembly and function.
• Tissue specificity: expressed in simple epithelia (e.g., gingival mucosa, hard palate) and in muscle fibers at costameres.
• Clinical relevance: associated with disease variants and host-virus interactions, highlighting its role in pathology.

Experimental Guidance and Technical Tips

• For intracellular flow cytometry, fix and permeabilize cells using optimized protocols (e.g., 4% PFA followed by 0.1% Triton X-100 or saponin-based buffers) to allow antibody access to the cytosolic keratin network.
• Titrate the -labeled antibody to determine the optimal staining concentration; typical starting dilutions are between 1:50 and 1:200, but variable by cell type.
• Use appropriate isotype control antibodies to set gating thresholds and confirm specificity of KRT8 staining.
• HeLa cells or other simple epithelial cell lines (e.g., MCF-7) can serve as positive controls due to high KRT8 expression; consider using knockout or knockdown cells as negative controls if available.
• Protect the antibody from prolonged light exposure to prevent fluorophore photobleaching during staining and acquisition.
• The immunogen spans nearly full-length KRT8 (aa 1-483), suggesting the antibody likely recognizes the native conformation; however, validation for other applications (e.g., WB, IHC) has not been provided and should be verified independently.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, supplying validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material solutions to diagnostic manufacturers, labs, and research institutes. This anti-cytokeratin 8 antibody fits seamlessly into epithelial cell research and cancer diagnostic development, complementing our cytoskeleton-related portfolio. From concept to clinic, we streamline your assay development with reliable supply and expert technical consulting.