Rabbit anti-Human Cytokeratin 19 (KRT19) Monoclonal Antibody for Flow Cytometry - P08727

Core Product Specifications and Parameters

Biological Background: Cytokeratin 19 Function and Localization

• Cytokeratin 19 (CK-19), also known as Keratin-19 (K19) and K1CS, is a type I intermediate filament protein encoded by the KRT19 gene.
• It plays a structural role in myofibers by linking the contractile apparatus to dystrophin at costameres, in coordination with KRT8.
• Expressed in a broad range of epithelial tissues: basal keratinocytes of hair follicles, sweat gland, mammary gland, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium.
• Also found in vascular smooth muscle cells, umbilical cord vascular walls, and striated muscle fibers where it localizes to costameres.
• Contains a central coiled-coil rod domain typical of keratins, facilitating intermediate filament assembly.
• Subject to post-translational phosphorylation and methylation, which may regulate filament dynamics.
• Has been identified in host-virus interaction pathways and is listed as a reference proteome member with direct protein sequencing evidence.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 301-400 of human Cytokeratin 19 (NP_002267.2). Consider verifying linear epitope accessibility in your target cell type.
• This antibody is directly conjugated to , making it suitable for intracellular flow cytometry. Use appropriate permeabilization and fixation protocols as required for nuclear/cytoplasmic antigens.
• Cross-reactivity is reported for human. If using with other species, validate specificity beforehand.
• Include relevant isotype controls and single-stain compensation controls in your flow cytometry panel design.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw material sourcing, offering a streamlined supply of validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents. This Cytokeratin 19 antibody supports research into epithelial biology and cancer stem cells. Our team can assist with bulk orders and rare target raw-material procurement to accelerate your diagnostic development.