Rabbit anti-Human Cytokeratin 18 Monoclonal Antibody for Intracellular Flow Cytometry (FC) - P05783

Core Product Specifications and Parameters

Biological Background: Cytokeratin 18 Function and Localization

• Keratin 18 (CK-18, K18) is a type I intermediate filament protein and the major keratin partner of type II keratin 8 (KRT8) in simple epithelial cells. It provides mechanical stability and resilience to epithelial tissues. Related references: PMID:15529338, PMID:26823019
• KRT8/KRT18 filaments mediate ARHGEF40-dependent actin stress fiber formation and reinforcement in response to tensional forces, and act downstream of ROCK kinase in a mechanical stress feedback loop. Related references: PMID:26823019
• The organized orientation of KRT8/KRT18 filaments contributes to the elongated morphology of epithelial tubules.
• Phosphorylation of KRT18 is critical for filament reorganization and dynamics. Related references: PMID:7523419, PMID:8522591, PMID:9298992
• KRT18 participates in the delivery of mutated CFTR to the plasma membrane and in interleukin-6 (IL-6)-mediated barrier protection. Related references: PMID:15529338, PMID:16424149, PMID:17213200
• It is involved in the hepatic uptake of thrombin-antithrombin complexes.
• Subcellularly, keratin 18 localizes to the nucleus matrix, nucleolus, perinuclear cytoplasm, and cytoplasmic intermediate filament networks.
• Tissue expression is prominent in colon, placenta, and liver, with weak expression in exocervix; expression increases in lymph nodes of breast carcinoma.

Experimental Guidance and Technical Tips

• Consider using this -conjugated antibody for intracellular flow cytometry detection of cytokeratin 18 in permeabilized human epithelial cells or carcinoma cell lines.
• Optimize fixation and permeabilization conditions for your specific sample type to maintain epitope recognition.
• Validate the antibody in your experimental system, as CK-18 expression levels can vary with cell differentiation and tissue origin.
• When multiplexing, ensure spectral compatibility of with other fluorophores in your panel.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a comprehensive portfolio of validated IVD raw materials, including monoclonal antibodies like this anti-cytokeratin 18 clone for intracellular flow cytometry. We streamline procurement with one-stop access to bulk reagents, antibody pairs, and customized sourcing solutions for epithelial cell biology and oncology biomarker development. Contact our team to discuss your project-specific requirements.