Rabbit anti-Human CR2/CD21 Monoclonal Antibody for FC - P20023

Core Product Specifications and Parameters

Biological Background: CR2/CD21 Function and Localization

• Complement receptor type 2 (CR2), also known as CD21, complement C3d receptor, and Epstein-Barr virus (EBV) receptor, is encoded by the CR2 gene and belongs to the family of complement regulators.
• Functions as a receptor for complement component C3d and other ligands including HNRNPU and IFNA1, facilitating antigen recognition and uptake by B-cells, which enhances B-cell activation and immune responses. Related references: PMID:1849076, PMID:21527715, PMID:7753047
• Forms a B-cell co-receptor complex with CD19, FCRL5, and CD81, crucial for B-cell activation and signaling. Related references: PMID:1383329, PMID:30107486
• Induces intracellular signaling independently of BCR and CD19 by activating tyrosine kinase SRC, leading to phosphorylation of nucleolin/NCL and triggering AKT and GSK3 kinase activities. Related references: PMID:12938232
• Acts as a ligand for CD23 (FcεRII), a low-affinity IgE receptor, thereby participating in the regulation of IgE production. Related references: PMID:1386409
• Serves as a receptor for Epstein-Barr virus, mediating viral entry into host cells.
• Highly expressed on mature B-lymphocytes, and also found on T-lymphocytes, pharyngeal epithelial cells, astrocytes, and follicular dendritic cells of the spleen.
• Localizes to the cell membrane and exists as multiple isoforms (110–119 kDa) due to alternative splicing; the protein is a glycoprotein containing disulfide bonds and multiple sushi (SCR) domains.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant fusion protein spanning nearly the entire extracellular domain (aa 21-971), likely preserving native conformation—consider using this antibody for native protein detection in flow cytometry.
• As a directly conjugated antibody, it enables one-step staining protocols; validate in the relevant sample system and include appropriate isotype controls.
• For flow cytometry, titrate the antibody on cells with known CD21 expression (e.g., B-cell lines or primary B-cells) to determine the optimal staining concentration.
• Since CD21 expression varies among lymphocyte subsets, include negative controls such as non-hematopoietic cells or CD21-knockdown samples to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing. For researchers and manufacturers focusing on complement biology and B-cell immunophenotyping, we connect you with robust supply chains for core reagents like anti-CR2/CD21 antibodies and related profiling tools. Let CamelBio accelerate your diagnostic development from concept to clinic.