Rabbit anti-Human CR2/CD21 Monoclonal Antibody for FC - P20023

Core Product Specifications and Parameters

Biological Background: CR2/CD21 Function and Localization

• CR2 functions as a receptor for complement component C3d, Epstein-Barr virus (EBV), and other ligands, mediating crucial roles in innate and adaptive immunity.
  Related references:
  PMID:1849076 PMID:21527715 PMID:7753047

• By binding C3d-coated antigens on B-cells, CR2 enhances antigen recognition and uptake, thereby facilitating B-cell activation and subsequent immune responses.
  Related references:
  PMID:21527715

• CR2 forms a B-cell coreceptor complex with CD19, CD81, and FCRL5, amplifying B-cell receptor (BCR) signaling and lowering the threshold for B-cell activation.
  Related references:
  PMID:1383329 PMID:30107486

• Independently of BCR and CD19, CR2 can activate SRC kinase, leading to nucleolin phosphorylation and downstream AKT/GSK3 signaling pathways.
  Related references:
  PMID:12938232

• CR2 acts as a ligand for CD23 (FcεRII), the low-affinity IgE receptor, implicating it in the regulation of IgE production.
  Related references:
  PMID:1386409

• CR2 is also the primary receptor for Epstein-Barr virus (EBV) entry into B-cells, facilitating viral infection and contributing to the pathogenesis of EBV-associated diseases.

• CR2 is a single-pass type I membrane protein characterized by an extracellular domain composed of 15–16 Sushi (CCP) repeats, and alternative splicing generates multiple isoforms.

• Tissue expression is predominantly on mature B-cells, but also on T-cells, pharyngeal epithelial cells, astrocytes, and follicular dendritic cells.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 21 to 971, encompassing the entire extracellular domain, which may enable recognition of native CR2/CD21 on live cells, making this antibody suitable for flow cytometry.
• For flow cytometry, consider titrating the antibody to determine the optimal concentration for your specific cell type, and always include appropriate isotype controls.
• CR2 expression is high on B-cells and follicular dendritic cells; validate in your target cell population using known positive and negative controls (e.g., peripheral blood B-cells vs. T-cells).
• Since CR2 serves as the EBV receptor, be aware that EBV-transformed lymphoblastoid cell lines may exhibit altered antigen expression levels.
• The antibody is conjugated to , a bright and photostable fluorophore similar to Alexa Fluor 594; consider this when designing multicolor panels to minimize spectral overlap.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw-material sourcing, from concept to clinic. For immunology and B-cell research, we offer validated antibody pairs, optimized monoclonal antibodies like this anti-CR2/CD21 clone, and bulk ancillary reagents. Our global supplier network also enables rapid sourcing of rare-target raw materials to accelerate your diagnostic development.