Rabbit anti-Human CLEC12A/CD371 Monoclonal Antibody for FC - Q5QGZ9

Core Product Specifications and Parameters

Biological Background: CLEC12A Function and Localization

• CLEC12A (CD371, CLL-1, MICL) is a single-pass type II transmembrane receptor of the C-type lectin family. Alternative splicing produces multiple isoforms.
• Functions as a myeloid inhibitory receptor; upon ligand engagement, its ITIM motif is phosphorylated, recruiting tyrosine-protein phosphatases PTPN6 and PTPN11 to dampen SYK-mediated activation signals, thereby suppressing myeloid cell responses including cytokine production and degranulation. Related references: PMID:14739280, PMID:16239426, PMID:34234773
• Acts as a pattern-recognition receptor for diverse ligands: recognizes DNA in neutrophil extracellular traps (NETs), monosodium urate crystals, and pathogen-associated molecules such as plasmodium hemozoin and mycobacterial mycolic acid, linking it to anti-inflammatory feedback and host defense. Related references: PMID:38367667, PMID:38386511, PMID:39143217, PMID:31269448, PMID:36542980
• Inhibits natural killer (NK) cell cytotoxicity and restrains dendritic cell maturation in an IL-10-dependent manner, contributing to immune homeostasis. Related references: PMID:15238421, PMID:16239426
• Localizes predominantly to the cell membrane.
• Preferentially expressed in lymphoid tissues and innate/adaptive immune cells, including NK cells, T cells, dendritic cells, monocytes, and macrophages; detected at the protein level in spleen macrophage-rich red pulp and lymph node. Related references: PMID:14739280, PMID:15238421, PMID:15548716, PMID:16239426, PMID:16838277
• Post-translational modifications include N-linked glycosylation and phosphorylation; the extracellular domain contains conserved disulfide bonds essential for structural integrity.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to the extracellular domain (aa 65-265) of human CLEC12A, which contains the C-type lectin domain and is sufficient for generating antibodies that recognize the native receptor on live cells.
• This conjugate is optimized for direct flow cytometry (FC) without a secondary antibody; consider titrating the antibody to determine the optimal working concentration for your cell type and staining conditions.
• For surface staining of CLEC12A, harvest cells and wash with FACS buffer (PBS containing 1–2% FBS or BSA). Incubate with the fluorophore-conjugated antibody at 2–8°C for 20–30 minutes, protected from light.
• Include appropriate isotype controls and Fc receptor blocking reagents to minimize non-specific binding, especially in myeloid cell populations.
• Data acquisition should be performed as soon as possible after staining; if delay is unavoidable, fix cells with paraformaldehyde after washing and protect from light.
• Avoid repeated freeze-thaw cycles of the antibody; store the conjugate undiluted at 2–8°C and protect from prolonged light exposure.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated B2B partner for IVD manufacturers and research laboratories, offering a streamlined supply of validated raw materials—from monoclonal antibodies and optimized antibody pairs to ancillary reagents and rare-target sourcing. This anti-CLEC12A/CD371 monoclonal antibody supports high-sensitivity flow cytometry for immune cell profiling, myeloid biology, and inflammation studies. Leverage CamelBio’s integrated sourcing capabilities to accelerate your assay development and secure consistent lot-to-lot performance for your diagnostic workflows.