Rabbit anti-Human Claudin18.2 Monoclonal Antibody for Flow Cytometry - P56856-2

Core Product Specifications and Parameters

Biological Background: Claudin18.2 Function and Localization

• Claudin18.2 is the isoform A2 of Claudin-18, encoded by the CLDN18 gene. It is a member of the claudin family of tight junction proteins.
• It is required for the formation of the gastric paracellular barrier, playing a critical role in the response to gastric acidification.
• Claudin18.2 localizes to tight junctions at the lateral cell membrane, contributing to cell polarity and barrier function.
• Expression is highly tissue-specific, restricted to the stomach mucosa. At the protein level, it is predominantly observed in epithelial cells of the pit region and the base of gastric glands, including both exocrine and endocrine cells.
• The protein has a molecular weight of approximately 27.7 kDa, consistent with the 27 kDa observed in SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein spanning amino acids 1-261 of human Claudin18.2 (NP_001002026.1). Consider this full-length extracellular domain coverage when designing detection strategies.
• The conjugate allows direct detection by flow cytometry (FC), eliminating the need for secondary antibodies. Ensure appropriate fluorophore compensation and use isotype controls.
• Validate antibody binding in a relevant cellular context, such as human gastric epithelial cell lines or primary stomach tissue samples, to confirm specificity.
• For optimal signal, titrate the antibody and validate using cells known to express Claudin18.2, as expression is restricted to stomach mucosa.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, contract research organizations, and academic laboratories a comprehensive sourcing channel for specialized IVD raw materials. This anti-Claudin18.2 monoclonal antibody exemplifies our access to rare-target reagents for gastric barrier and tight junction research. From validated antibody pairs to bulk ancillary reagents, CamelBio streamlines your development pipeline with reliable, one-stop procurement support.