Rabbit anti-Human Claudin18.2 Monoclonal Antibody for FC - P56856-2

Core Product Specifications and Parameters

Biological Background: Claudin18.2 Function and Localization

• Claudin-18 isoform A2 (Claudin18.2) is encoded by the CLDN18 gene and is a key tight junction protein in the stomach.
• It is required for gastric paracellular barrier formation, modulating permeability in response to acid.
• Localizes to tight junctions and lateral cell membranes of gastric epithelial cells.
• Tissue expression is highly specific to the stomach mucosa, detected in epithelial cells of the pit region and base of gastric glands, including exocrine and endocrine cells.
• Predicted molecular weight is approximately 27.7 kDa.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-261) of Claudin18.2, which likely includes extracellular domains; the antibody may recognize surface-expressed Claudin18.2, making it suitable for live-cell flow cytometry.
• For flow cytometry, titrate the antibody on your target cells to determine the optimal staining concentration. Include appropriate isotype controls and validate specificity using Claudin18.2-negative cells if available.
• Ensure single-cell suspension and proper viability staining; the conjugate allows direct detection without secondary reagents.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with reliable access to high-quality IVD antibodies, including this anti-Claudin18.2 monoclonal antibody for gastric biology and tight junction studies. As your one-stop sourcing bridge, we offer validated antibody pairs, bulk reagents, and custom solutions to accelerate your assay development from concept to clinic.