Rabbit anti-Human CEACAM6 Monoclonal Antibody for Flow Cytometry - P40199

Core Product Specifications and Parameters

Biological Background: CEACAM6 Function and Localization

• CEACAM6 (CD66c, NCA, CEAL) is a GPI-anchored cell surface glycoprotein belonging to the immunoglobulin superfamily and the carcinoembryonic antigen (CEA) family.
• It mediates calcium- and fibronectin-independent intercellular adhesion through homophilic interactions and heterophilic binding with CEACAM5 and CEACAM8. Related references: PMID:11590190 PMID:16204051 PMID:2022629
• Heterophilic interaction with CEACAM8 on activated neutrophils facilitates neutrophil adhesion to cytokine-activated endothelial cells. Related references: PMID:8776764 PMID:1378450
• CEACAM6 plays a role in cell migration and tumor progression, with overexpression observed in many cancer cell lines. Related references: PMID:16204051 PMID:10910050
• It is localized predominantly at the cell membrane, including the apical surface of polarized epithelial cells, and on the cell surface.
• Tissue expression includes neutrophils, columnar epithelial and goblet cells of the colon, and numerous tumor cell lines.

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular domain (aa 35–320) of human CEACAM6, suggesting recognition of native surface-expressed protein; consider using standard cell surface staining protocols for flow cytometry without permeabilization.
• excites at 488 nm, compatible with most flow cytometers; perform antibody titration to determine the optimal working concentration.
• Appropriate positive controls include human peripheral blood neutrophils, granulocytes, or colon carcinoma cell lines (e.g., HT-29, Caco-2) that express CEACAM6.
• For multicolor panels, check for potential spectral overlap and ensure proper compensation controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by offering a comprehensive portfolio of high-quality antibodies, antigens, and ancillary reagents. This -conjugated anti-CEACAM6 monoclonal antibody supports oncology and inflammation research, where CEACAM6 serves as a cell adhesion and tumor marker. Backed by reliable supply capabilities, CamelBio enables diagnostic developers and laboratories to source validated antibody pairs, bulk monoclonal/polyclonal antibodies, and rare-target raw materials efficiently.