Rabbit anti-Human CEACAM5/CD66e Monoclonal Antibody for FC - P06731

Core Product Specifications and Parameters

Biological Background: CEACAM5 Function and Localization

• CEACAM5 (CD66e, CEA) is a cell surface glycoprotein that mediates homophilic and heterophilic cell adhesion with other CEACAM family members, such as CEACAM6. Related references: PMID:2803308
• It functions as an oncogene, promoting tumor progression and inducing resistance to anoikis in colorectal carcinoma cells. Related references: PMID:10910050
• Intracellular signaling mediated by CEACAM5 contributes to its role in cell adhesion and tumor progression. Related references: PMID:10864933
• Acts as a receptor for E.coli Dr adhesins during microbial infection, leading to dissociation of the CEACAM5 homodimer.
• Localizes to the cell membrane, primarily the apical cell membrane in polarized cells, and is anchored via a GPI moiety (UniProt keyword: GPI-anchor).
• Expressed in columnar epithelial and goblet cells of the normal colon, and highly expressed in adenocarcinomas of the digestive system and fetal colon. Related references: PMID:10436421
• Contains immunoglobulin domains and multiple disulfide bonds, characteristic of the CEACAM family, and undergoes alternative splicing (UniProt keywords: Immunoglobulin domain, Disulfide bond, Alternative splicing).
• Post-translational modifications include glycosylation (UniProt keyword: Glycoprotein) and attachment to the membrane via a GPI anchor (Lipoprotein).

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal domain excluding the signal peptide (aa 35-685), which includes the extracellular IgG-like domains; thus the antibody likely recognizes an extracellular epitope suitable for live cell flow cytometry.
• For flow cytometry, consider titrating the antibody to determine optimal concentration and use appropriate isotype controls. Include viability dyes to exclude dead cells.
• As a GPI-anchored protein, CEACAM5 may be released by phosphatidylinositol-specific phospholipase C (PI-PLC) treatment; consider this when validating staining under different conditions.
• Cross-reactivity is reported for human; validate reactivity in relevant human cell lines (e.g., colorectal adenocarcinoma) or primary samples.
• Avoid using antibodies conjugated to fluorophores that may be sensitive to light or temperature extremes; store and handle according to the manufacturer's recommendations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For CEACAM5/CD66e, a key tumor marker and oncogene, we offer well-characterized monoclonal antibodies like this conjugate optimized for flow cytometry, alongside sourcing of complementary reagents, antibody pairs, and ancillary materials to support your assay development.