Rabbit anti-Human CDCP1 Monoclonal Antibody for FC - Q9H5V8

Core Product Specifications and Parameters

Biological Background: CDCP1 Function and Localization

• CDCP1 (CUB domain-containing protein 1), also known as CD318, TRASK, and SIMA135, is a transmembrane glycoprotein and member of the tetraspanin web.
• It is involved in cell adhesion and cell matrix association, potentially regulating anchorage-dependent migration and proliferation-differentiation balance via phosphorylation.
• CDCP1 has been proposed as a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets.
• It is implicated in tumor progression and metastasis as part of the tetraspanin web.
• Subcellularly, CDCP1 localizes to the cell membrane and is also secreted.
• The protein is highly expressed in mitotic cells, with lower levels in interphase; it shows highest tissue expression in skeletal muscle and colon, with lower levels in kidney, small intestine, placenta, and lung.
• CDCP1 is up-regulated in multiple human cancers including colorectal, breast, and lung carcinomas, and is expressed in mesenchymal and neural stem-like cells.
• Key post-translational modifications include phosphorylation, glycosylation, and disulfide bond formation; the protein undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain of CDCP1 (aa 30-666), suggesting detection of both membrane-bound and secreted forms.
• This antibody is conjugated to , a green fluorescent dye, for direct flow cytometry.
• For FC, consider titrating the antibody to determine optimal concentration for your cell type, and include appropriate isotype controls.
• Since CDCP1 is expressed on the cell surface, live-cell staining is recommended; avoid permeabilization if only surface staining is desired.
• Validate staining in known positive cell lines (e.g., from colon or lung carcinoma) and check expression in mitotic versus interphase cells.

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CamelBio is your one-stop sourcing partner for high-quality IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Whether you are developing assays for cancer diagnostics or stem cell research, we support your needs from concept to clinic.