Rabbit anti-Human CDA Monoclonal Antibody for Intracellular Flow Cytometry - P32320

Core Product Specifications and Parameters

Biological Background: Cytidine Deaminase Function and Localization

• Cytidine deaminase (CDA), also referred to as CDD, is an enzyme encoded by the CDA gene in humans.
• It catalyzes the deamination of exogenous and endogenous cytidine and 2'-deoxycytidine, funneling them into UMP synthesis as part of the pyrimidine salvage pathway.
• The enzyme belongs to the hydrolase family and requires zinc as a cofactor for its catalytic activity, as indicated by its metal-binding properties.
• CDA expression is largely restricted to granulocytes, with minimal expression in fibroblasts, chondrocytes, monocytes, and T- and B-cell lines.

Experimental Guidance and Technical Tips

• As the immunogen comprises amino acids 1-146 of human CDA, the resulting monoclonal antibody recognizes the N-terminal region of the native protein; careful epitope mapping or competition assays may help confirm target specificity in complex samples.
• This antibody is validated for intracellular flow cytometry; ensure appropriate permeabilization and fixation protocols are optimized for the cell type of interest to achieve reliable staining.
• Given the high expression in granulocytes and low expression in lymphocytes, consider using a granulocyte-rich sample (e.g., neutrophil preparations) as a positive control and lymphocyte populations as a negative reference.
• Since the conjugate is a bright fluorophore (), titrate the antibody to minimize background and achieve optimal signal-to-noise ratio; include an isotype control to assess non-specific binding.
• For multicolor panels, verify compatibility of with other fluorophores and check for potential spectral overlap.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive sourcing solutions for diagnostic manufacturers and research laboratories, supplying validated monoclonal antibodies, recombinant proteins, and ancillary reagents for IVD development. Focusing on targets like CDA involved in nucleotide metabolism, we provide one-stop access to optimized raw materials, technical consultation, and customizable supply chains to accelerate your assay development from concept to clinic. Whether you need high-quality conjugated antibodies for flow cytometry or bulk quantities of specialized reagents, CamelBio bridges your research needs with reliable, scalable sourcing.