Rabbit anti-Human CDA Monoclonal Antibody for FC (intra) - P32320

Core Product Specifications and Parameters

Biological Background: CDA Function and Localization

• Cytidine deaminase (CDA, also known as Cytidine aminohydrolase or CDD) is a zinc-dependent hydrolase that catalyzes the deamination of cytidine and 2'-deoxycytidine to uridine and 2'-deoxyuridine, respectively.
• This enzyme plays a key role in pyrimidine salvage, scavenging exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis, thereby supporting nucleotide metabolism.
• CDA is predominantly expressed in granulocytes, with very low levels detected in fibroblasts, chondrocytes, monocytes, and T- and B-cell lines, indicating a tissue-specific role in myeloid cells.
• The enzyme is a member of the cytidine deaminase family and requires zinc as a cofactor for its catalytic activity (UniProt keywords: Metal-binding, Zinc).
• The human CDA gene (GeneID: 978) encodes a 146-amino acid protein with a predicted molecular weight of approximately 16 kDa (UniProt: 16185 Da).
• CDA has been structurally characterized (3D-structure available) and is listed as a reference proteome component, with proteomics identification confirming its expression.

Experimental Guidance and Technical Tips

• For intracellular flow cytometry, ensure proper fixation and permeabilization protocols to allow antibody access to the cytoplasmic CDA protein.
• The immunogen encompassing the full-length (1-146) human CDA suggests the antibody may recognize both denatured and native conformations; however, validation in the specific experimental context is recommended.
• Given the high expression in granulocytes, consider using granulocyte-rich samples (e.g., whole blood or bone marrow) as positive controls, while fibroblasts or lymphocytes may serve as negative controls.
• Cross-reactivity with other species has not been reported; thus, when using non-human samples, validate the antibody's reactivity beforehand.
• The conjugate allows direct detection by flow cytometry; titrate the antibody to optimize signal-to-noise ratio for your cell type of interest.
• Store the antibody as recommended (ice bag shipping suggests storage at 2-8°C or frozen aliquots) and avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined sourcing experience for IVD raw materials, including validated antibodies, antigen pairs, and ancillary reagents. This Rabbit anti-Human CDA mAb exemplifies our quality monoclonal antibody portfolio, supporting nucleotide metabolism and immunological research. Our platform also assists in rare target sourcing and bulk supply, bridging the gap from concept to clinical application.