Rabbit anti-Human CD99 Monoclonal Antibody for IF/ICC, FC - P14209

Core Product Specifications and Parameters

Biological Background: CD99 Function and Localization

• CD99 (Cluster of Differentiation 99), also known as MIC2, E2 antigen, or T-cell surface glycoprotein E2, is a transmembrane glycoprotein encoded by the CD99 gene.
• It mediates T-cell adhesion processes and facilitates spontaneous rosette formation with erythrocytes (by similarity).
• CD99 contributes to leukocyte extravasation by enabling cells to cross the endothelial basement membrane, functioning at the same step as PECAM1 but independently.
• The protein is located at the plasma membrane, featuring a type I topology with a signal peptide, extracellular domain, single transmembrane helix, and cytoplasmic tail.
• CD99 undergoes alternative splicing and is subject to post-translational modifications including glycosylation and phosphorylation.
• The UniProt curated entry includes 3D structural data and direct protein sequencing evidence, with a predicted molecular weight of approximately 18.8 kDa.

Experimental Guidance and Technical Tips

• The immunogen (aa 24-123) corresponds to an extracellular region of CD99, suggesting the antibody may recognize an extracellular epitope and could be suitable for live-cell staining without permeabilization.
• For immunofluorescence (IF)/immunocytochemistry (ICC), standard methanol or formaldehyde fixation is typically used; consider optimizing fixation and permeabilization protocols for your specific cell type.
• For flow cytometry (FC), the conjugate is ready to use; perform antibody titration to determine the optimal concentration for your cellular system and target expression level.
• This antibody is validated for human CD99; when working with other species, verify the sequence homology of the immunogen region to assess potential cross-reactivity.
• The detected protein weight is 19 kDa; if additional validation by western blot is required, note that this monoclonal antibody may require testing under denaturing conditions, as WB is not a listed application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to provide one-stop access to IVD raw materials. This anti-CD99 monoclonal antibody supports investigations in leukocyte adhesion and transmigration; beyond this product, CamelBio supplies validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to accelerate your development timelines. Contact us for bulk purchasing or custom conjugation services.