Rabbit anti-Human CD99 Monoclonal Antibody for IF/ICC and FC - P14209

Core Product Specifications and Parameters

Biological Background: CD99 Antigen Function and Localization

• CD99 antigen (also known as MIC2, E2 antigen, 12E7, or T-cell surface glycoprotein E2) is a transmembrane protein encoded by the CD99 gene in humans.
• It functions in T-cell adhesion processes, including spontaneous rosette formation with erythrocytes, a characteristic of T-cells.
• CD99 plays a critical role in leukocyte extravasation, facilitating the passage of leukocytes through the endothelial basement membrane at a late step, acting independently of PECAM1.
• The protein is located at the plasma membrane (single-pass type I membrane protein) with its N-terminus exposed extracellularly.
• Post-translational modifications include glycosylation and phosphorylation (keywords: Glycoprotein, Phosphoprotein).
• Alternative splicing generates multiple isoforms, and the protein is part of the reference proteome with direct protein sequencing evidence.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 24-123 of human CD99, corresponding to the N-terminal extracellular region. This design likely targets an exposed epitope, suitable for live-cell staining applications.
• For immunofluorescence (IF/ICC), fixation with paraformaldehyde is recommended, but permeabilization may be omitted if the epitope is accessible on the cell surface. Test staining with and without detergent to confirm.
• In flow cytometry (FC), this conjugate enables direct detection; consider using appropriate isotype controls and titrating the antibody for optimal signal-to-noise ratio.
• Validate the antibody's specificity in your experimental system using CD99-positive cell lines (e.g., T-cell lines) and CD99-negative controls to confirm minimal cross-reactivity.
• As CD99 is involved in adhesion, ensure that cell harvesting methods do not damage surface antigens; gentle detachment techniques are advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material partner, supplying validated antibodies, bulk ancillary reagents, and customized sourcing solutions. From early-stage research to diagnostic development, we support projects in immunology, including CD99-mediated adhesion and leukocyte trafficking. Contact us to explore tailored antibody panels, large-scale production, and rare target material procurement.