Rabbit anti-Human CD99 mAb for FC - P14209

Core Product Specifications and Parameters

Biological Background: CD99 Function and Localization

• CD99 antigen, also known as MIC2, E2 antigen, or T-cell surface glycoprotein E2, is a type I transmembrane glycoprotein encoded by the CD99 gene (MIC2, MIC2X, MIC2Y) in humans.
• It mediates T-cell adhesion processes and spontaneous rosette formation with erythrocytes. Additionally, CD99 plays a critical role in leukocyte extravasation, facilitating leukocyte passage through the endothelial basement membrane independently of PECAM1 (By similarity).
• The protein is predominantly localized to the plasma membrane.
• CD99 is a phosphoprotein and glycoprotein, indicating post-translational modifications that may regulate its function.
• Alternative splicing generates multiple isoforms of CD99, contributing to functional diversity.
• The protein has a determined 3D structure and is part of the reference proteome, with direct protein sequencing evidence.
• Keywords associated with CD99 include cell adhesion, membrane, and proteomics identification, reflecting its role in cellular interactions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 24-123 of human CD99. For flow cytometry, consider titrating the antibody to determine the optimal concentration for your cell type, balancing staining intensity and background.
• This antibody is intended for flow cytometry applications. Ensure proper cell preparation, including Fc receptor blocking and live/dead discrimination, to enhance specificity.
• Include appropriate isotype controls and compare with known positive and negative cell lines to confirm CD99 expression in your experimental system.
• When studying T-cell adhesion or leukocyte extravasation, validate CD99 surface expression under your specific culture or treatment conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a comprehensive, one-stop solution for IVD raw materials, technical services, and consulting—supporting every stage from concept to clinic. For CD99-related immunoassay development, CamelBio provides validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to streamline your workflow. With expertise in sourcing rare targets and custom raw-material procurement, CamelBio serves as a reliable partner to advance your T-cell adhesion and leukocyte extravasation research.