Rabbit anti-Human CD94 Monoclonal Antibody for Flow Cytometry - Q13241

Core Product Specifications and Parameters

Biological Background: CD94 Function and Localization

• CD94 (KLRD1) is a C-type lectin-like receptor that serves as the ligand-binding subunit of the CD94/NKG2 heterodimeric receptor complexes on natural killer (NK) cells and subsets of T cells. It lacks intrinsic signaling capacity, relying on its partner chains KLRC1 (NKG2A) or KLRC2 (NKG2C) for signal transduction.
• The CD94/NKG2A complex functions as an inhibitory receptor, recognizing the non-classical MHC class Ib molecule HLA-E bound to self-peptides derived from classical MHC class I signal sequences. This interaction enables NK cells to monitor MHC class I expression and maintain self-tolerance. Related references: PMID:10023772, PMID:18064301, PMID:18083576
• The CD94/NKG2C complex acts as an activating receptor, recognizing HLA-E loaded with peptides from HLA-G, and plays roles in adaptive NK cell responses and maternal-fetal tolerance. Related references: PMID:15940674, PMID:9655483, PMID:30134159, PMID:9754572, PMID:20952657, PMID:21825173
• CD94 is expressed on the cell membrane of NK cells, memory/effector CD8+ αβ T cells, and terminally differentiated γδ T cells, with differential expression of NKG2A and NKG2C in these populations.
• As a glycoprotein with a transmembrane domain and disulfide bonds, CD94 is classified as a receptor with innate and adaptive immunity roles, and it is involved in host-virus interactions.
• In viral infections, CD94/NKG2A can mediate immune evasion. During SARS-CoV-2 infection, CD94 may recognize HLA-E presenting viral spike protein peptides on lung epithelial cells, leading to NK cell exhaustion and reduced antiviral surveillance. Related references: PMID:32859121

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region of CD94, suggesting the antibody binds a surface-exposed epitope.
• For flow cytometry, consider using human peripheral blood mononuclear cells or NK cell lines, and validate binding in the relevant sample system.
• Ensure optimization of antibody titration and appropriate isotype controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material procurement. This CD94 antibody supports immune checkpoint and NK cell research, and CamelBio’s expert team can assist with tailored supply solutions to accelerate your diagnostic development from concept to clinic.