Rabbit anti-Human CD93 Monoclonal Antibody for Flow Cytometry - Q9NPY3

Core Product Specifications and Parameters

Biological Background: CD93 Function and Localization

• CD93, also known as Complement component C1q receptor (C1qR, C1qRp), is a cell surface receptor encoded by the CD93 gene. It is a transmembrane glycoprotein belonging to the Group XIV C-type lectin family. Related references: PMID:7977768
• It functions as a receptor for defense collagens, including surfactant protein A (SFTPA1), complement component C1q, and mannose-binding lectin (MBL2), thereby enhancing phagocytosis of apoptotic cells and immune complexes. Related references: PMID:7977768
• CD93 plays a critical role in angiogenesis by regulating endothelial cell adhesion and migration. It associates with beta-dystroglycan to activate SRC kinase, leading to CBL recruitment and CRKL-mediated signaling. Related references: PMID:24809468 PMID:26848865
• It also serves as a receptor for pan-endothelial glycoprotein multimerin-2 (MMRN2) and IGFBP7, both of which contribute to CD93-mediated endothelial functions. Related references: PMID:28671670 PMID:36265539 PMID:38218180
• CD93 is highly expressed in endothelial cells, platelets, and myeloid cells (monocytes, neutrophils), but not in lymphoid cells, indicating a role in innate immunity and vascular biology.
• The protein undergoes post-translational modifications including phosphorylation, glycosylation, and disulfide bond formation, essential for its stability and function. It contains EGF-like domains.
• Keywords: Cell adhesion, Membrane receptor, Lectin, Host-virus interaction, Angiogenesis, Inflammatory response.

Experimental Guidance and Technical Tips

• This -conjugated antibody is optimized for direct flow cytometry detection of CD93 on live, unfixed cells. Since CD93 is a cell surface receptor, avoid permeabilization steps unless intracellular staining is required.
• Validate antibody performance in your specific cell type of interest. Positive controls include human endothelial cells, monocytes, or neutrophils, where CD93 is highly expressed; lymphoid cells serve as negative controls.
• Use an appropriate isotype control conjugated to the same fluorophore to assess nonspecific binding. Titrate the antibody to determine the optimal concentration for your assay.
• For dual-color flow cytometry, ensure spectral compatibility of with other fluorophores used. Note that has excitation/emission similar to Alexa Fluor 594.
• The immunogen spans amino acids 24-580, covering nearly the entire extracellular domain, which may enhance recognition of native CD93.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers and researchers with reliable IVD raw materials, including this high-quality anti-CD93 monoclonal antibody. We support your CD93-related studies—from inflammation and angiogenesis to immune complex clearance—with validated antibody pairs, bulk ancillary reagents, and expert sourcing for rare targets. Contact us to streamline your procurement from concept to clinic.