Rabbit anti-Human CD90/Thy1 Monoclonal Antibody for Flow Cytometry - P04216

Core Product Specifications and Parameters

Biological Background: CD90/Thy1 Function and Localization

• Thy-1 membrane glycoprotein, also known as CD90 or CDw90, is a cell surface protein encoded by the THY1 gene.
• It is anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor, classifying it as a GPI-anchored glycoprotein.
• Thy-1 contains an immunoglobulin-like domain and belongs to the immunoglobulin superfamily.
• The protein is implicated in cell-cell and cell-ligand interactions, particularly during synaptogenesis and other events in the brain.
• It is expressed on various cell types, including neurons, thymocytes, and subsets of fibroblasts and stem cells.
• Post-translational modifications include a disulfide bond, N-linked glycosylation, and a GPI-anchor attachment; it also contains a signal peptide and a pyrrolidone carboxylic acid modification.
• The mature protein has a theoretical molecular weight of approximately 18 kDa (17935 Da), but glycosylation may increase the apparent size in SDS-PAGE.
• UniProt keywords: Cell membrane, Disulfide bond, Glycoprotein, GPI-anchor, Immunoglobulin domain, Lipoprotein, Membrane, Phosphoprotein, Proteomics identification, Pyrrolidone carboxylic acid, Reference proteome, Signal.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal staining concentration for your cell type.
• Use appropriate isotype controls and include Fc receptor blocking if needed.
• The antibody recognizes an extracellular epitope within amino acids 20-130, suitable for live cell staining without permeabilization.
• As a monoclonal antibody, specificity is high; nonetheless, validate performance in your specific sample system.
• Ensure cells are properly suspended and avoid aggregation for accurate flow cytometric analysis.
• Protect the antibody from light and store at 4°C for short-term use; avoid repeated freeze-thaw cycles.
• Consider that the glycosylated mature protein may migrate at a higher molecular weight than the predicted 18 kDa.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable one-stop partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For CD90/Thy1 research in neuroscience and immunology, our sourcing services can help you access rare-target reagents and custom development support. Let CamelBio streamline your diagnostic development from concept to clinic.