Rabbit anti-Human CD9 Monoclonal Antibody for Flow Cytometry - P21926

Core Product Specifications and Parameters

Biological Background: CD9 Function and Localization

• CD9, also designated as MIC3, MRP-1, BTCC-1, DRAP-27, and TSPAN29, belongs to the tetraspanin superfamily of integral membrane proteins.
• It mediates sperm-egg fusion, platelet activation and aggregation, and regulates cell adhesion and motility, thereby influencing tumor metastasis. Related references: PMID:14575715, PMID:18541721, PMID:8478605, PMID:7511626
• CD9 associates with integrins and other tetraspanins such as CD81 to modulate cell fusion processes, including macrophage multinucleation and myotube formation. Related references: PMID:12796480
• The protein localizes to the plasma membrane and is also found in secreted extracellular exosomes.
• Post-translational modifications include N-linked glycosylation and palmitoylation, with disulfide bonds contributing to structural stability.
• CD9 is predominantly detected in platelets and broadly expressed in hematopoietic and epithelial cells. Related references: PMID:19640571

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1–228 of human CD9, encompassing the large extracellular domain, which is the primary antigenic region for antibody recognition.
• Direct conjugation to eliminates the need for secondary antibodies in flow cytometry, reducing background and protocol steps.
• Consider titrating the antibody and including Fc receptor blocking in samples rich in macrophages or granulocytes.
• Validate staining with relevant positive controls such as platelets, peripheral blood mononuclear cells, or CD9-expressing cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, supporting diagnostic developers with validated antibody pairs, bulk ancillary reagents, and rare target sourcing. This anti-CD9 monoclonal antibody, suited for hematology and oncology research, exemplifies our commitment to delivering high-quality, ready-to-use reagents for flow cytometry and other immunoassays. Partner with us to streamline your supply chain from concept to clinic.