Rabbit anti-Human CD8b Monoclonal Antibody for Flow Cytometry - P10966

Core Product Specifications and Parameters

Biological Background: CD8b Function and Localization

• T-cell surface glycoprotein CD8 beta chain (CD8b) is an integral membrane glycoprotein that serves as a coreceptor for MHC class I molecules in T-cells.
• CD8b interacts simultaneously with the T-cell receptor (TCR) and MHC class I:peptide complex on antigen-presenting cells, recruiting the Src kinase LCK to the TCR-CD3 complex to initiate signaling.
• The cytoplasmic tail of CD8b contains a palmitoylation site that contributes to partitioning of CD8 into plasma membrane lipid rafts, where signaling proteins are enriched.
• CD8b plays a critical role in thymic selection of CD8+ T-cells and is essential for cytotoxic T-lymphocyte (CTL) activation, leading to lymphokine production, motility, adhesion, and target cell killing.
• Multiple isoforms (isoforms 1, 3, 5, 6, 7, and 8) are expressed in thymus and peripheral CD8+ T-cells, with isoform 1 higher in thymus and isoform 6 higher in peripheral T-cells.
• The protein belongs to the immunoglobulin superfamily, contains a signal peptide, a transmembrane helix, and is subject to glycosylation and disulfide bond formation.
• Keywords: Adaptive immunity, Alternative splicing, Cell membrane, Glycoprotein, Immunity, Lipoprotein, Palmitate, Phosphoprotein, Secreted.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 22-170), suggesting the antibody likely recognizes surface-expressed CD8b on live cells, suitable for flow cytometry without permeabilization.
• For flow cytometry, titrate the antibody to determine the optimal staining concentration for your specific cell type and protocol.
• Include appropriate isotype controls and viability dyes to ensure accurate gating of CD8b+ populations.
• Ensure proper compensation settings when using with other fluorophores, as spectral overlap may occur.
• Validate the antibody in the relevant sample system (e.g., human PBMCs) to confirm expected CD8+ T-cell staining patterns.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials and technical services, covering every stage from concept to clinic. For immunology and T-cell research, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. This anti-CD8b antibody is part of our immunophenotyping portfolio; contact us to discuss your custom IVD development needs.