Rabbit anti-Human CD8b Monoclonal Antibody for FC - P10966

Core Product Specifications and Parameters

Biological Background: CD8b Function and Localization

• T-cell surface glycoprotein CD8 beta chain (CD8b) is an integral membrane glycoprotein essential for adaptive immunity, acting as a coreceptor for MHC class I:peptide complexes on antigen-presenting cells.
• CD8b pairs with CD8a to form the CD8 coreceptor on cytotoxic T lymphocytes, recognizing antigens presented by MHC class I molecules and initiating immune responses against intracellular pathogens.
• Upon TCR ligation, CD8b recruits the Src kinase LCK via its cytoplasmic tail, triggering downstream phosphorylation cascades that promote T-cell activation, motility, and cytotoxic function.
• A palmitoylation site in the cytoplasmic tail targets CD8b to plasma membrane lipid rafts, concentrating key signaling intermediates for efficient signal transduction.
• CD8b is critical for thymic selection, ensuring the maturation of functional CD8+ T-cells and contributing to central tolerance.
• Multiple isoforms arise from alternative splicing; isoform 1 is predominantly expressed in thymic CD8+ T-cells, whereas isoform 6 shows higher levels in peripheral CD8+ T-cells. Secreted isoforms have also been detected.
• The protein is a member of the immunoglobulin superfamily, featuring a transmembrane helix, disulfide bonds, glycosylation, and phosphorylation sites.

Experimental Guidance and Technical Tips

• This antibody is validated for flow cytometry. Titrate to determine the optimal working concentration for your specific cell population and staining protocol.
• The immunogen (aa 22–170) covers the extracellular region of CD8b, suggesting suitability for surface staining on live or fixed cells without permeabilization.
• Use appropriate isotype and fluorescence-minus-one (FMO) controls to distinguish specific from nonspecific staining, especially when analyzing rare T-cell subsets.
• For multiplex immunophenotyping, combine this anti-CD8b antibody with markers such as CD3, CD4, and CD8a to delineate cytotoxic T-cell populations effectively.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to a wide range of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies like this anti-CD8b clone, and bulk ancillary reagents. Specializing in immunology targets, we support development of T-cell-based diagnostics and immune monitoring assays. From concept to clinic, CamelBio is your one-stop partner for reliable raw-material sourcing and technical support.