Rabbit anti-Human CD89 Monoclonal Antibody for FC - P24071

Core Product Specifications and Parameters

Biological Background: CD89 Function and Localization

• CD89, also known as Fc alpha receptor (FcalphaR), is a member of the immunoglobulin superfamily and functions as a receptor for IgA.
• The protein is encoded by the FCAR gene and exists in several isoforms (A.1, A.2, A.3) generated by alternative splicing, with differential expression in blood and mucosal myeloid cells.
• CD89 primarily binds the Fc region of IgA, triggering immune responses such as cytokine production, phagocytosis, and antibody-dependent cell-mediated cytotoxicity.
• It is localized on the cell membrane of myeloid cells, including monocytes, neutrophils, and macrophages, and can also be found in secreted forms.
• Isoform A.1 and A.2 are expressed in alveolar macrophages, while all three isoforms are expressed in monocytes; surface expression is isoform-specific.
• The receptor contains immunoglobulin-like domains, a transmembrane helix, and a cytoplasmic tail, and is involved in immune regulation and mucosal immunity.
• Keywords from UniProt include disulfide bond, glycoprotein, signal peptide, and transmembrane, indicating post-translational modifications and membrane association.

Experimental Guidance and Technical Tips

• The recombinant protein immunogen (full-length human CD89) ensures high specificity for native antigen detection; confirm expression in your target cell type.
• This antibody is validated for flow cytometry (FC); optimize staining conditions, including antibody titration and cell fixation/permeabilization protocols, in your experimental system.
• Use appropriate isotype controls and ensure compensation settings when using fluorophore-conjugated antibodies for multicolor FC.
• Cross-reactivity is reported against human; verify reactivity in other species if required.
• The predicted protein weight is 32 kDa; however, glycosylation may result in higher observed molecular weight; validate in relevant sample lysates if using in other applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers a seamless sourcing experience for IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies like this anti-CD89 reagent. Our portfolio covers immune receptor targets, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development from concept to clinic.