Rabbit anti-Human CD89 Monoclonal Antibody for FC - P24071

Core Product Specifications and Parameters

Biological Background: CD89 Function and Localization

• CD89, also known as Immunoglobulin alpha Fc receptor, is a transmembrane glycoprotein that serves as the receptor for the Fc region of immunoglobulin A (IgA). It is encoded by the FCAR gene and belongs to the immunoglobulin superfamily.
• The protein contains two extracellular immunoglobulin-like domains, a single transmembrane helix, and a short cytoplasmic tail. It is extensively glycosylated and contains disulfide bonds that stabilize its structure.
• CD89 is expressed primarily on the surface of myeloid cells, including monocytes, macrophages, neutrophils, and eosinophils. This receptor mediates immune responses such as phagocytosis, antibody-dependent cellular cytotoxicity (ADCC), and release of inflammatory mediators upon binding IgA immune complexes.
• Alternative splicing generates multiple isoforms (A.1, A.2, A.3) that show differential expression patterns; for instance, isoform A.1 and A.2 are found on alveolar macrophages, while A.1, A.2, and A.3 are expressed in monocytes.
• A soluble form of CD89 can be released from the cell surface by proteolytic cleavage, and it is found in secretions. This soluble receptor may modulate IgA-mediated responses systemically.
• CD89 plays a crucial role in mucosal immunity and systemic IgA homeostasis. Dysregulation of CD89 function has been implicated in various autoimmune and inflammatory diseases.

Experimental Guidance and Technical Tips

• This product is a directly conjugated antibody, eliminating the need for a secondary antibody and reducing non-specific binding. It is recommended for flow cytometry analysis.
• The immunogen covers the extracellular domain (aa 22–227) of human CD89, suggesting the antibody binds to an epitope accessible on live cells without permeabilization.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific experimental system. Use appropriate isotype control and Fc blocking to minimize background.
• Validate the antibody in your target cell type (e.g., monocytes, neutrophils) as expression levels can vary. Include unstained and single-color controls for multicolor panels.
• Ensure cells are kept at 4°C and in buffer containing protein (e.g., 1% BSA) to maintain viability and reduce non-specific staining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material supply. This Rabbit anti-Human CD89 mAb supports research in immunology and diagnostic development, particularly for IgA receptor studies. With CamelBio's comprehensive procurement solutions, you can accelerate the transition from concept to clinic.