Rabbit anti-Human CD87/PLAUR Monoclonal Antibody for Flow Cytometry - Q03405

Core Product Specifications and Parameters

Biological Background: CD87/PLAUR Function and Localization

• Urokinase plasminogen activator surface receptor (uPAR, CD87) is a GPI-anchored glycoprotein encoded by the PLAUR gene, expressed on various cell types including immune cells and tumor cells.
• Acts as a high-affinity receptor for urokinase-type plasminogen activator (uPA), leading to localized plasmin generation and subsequent extracellular matrix (ECM) degradation, facilitating cell migration and tissue remodeling. Related references: PMID:1689240 PMID:15677461
• Independently binds the ECM protein vitronectin, promoting cell adhesion and indirectly activating integrin signaling pathways, which coordinate cell proliferation and survival. Related references: PMID:17548516 PMID:25168639 PMID:28849762
• Found at the cell membrane, particularly enriched at invadopodia—actin-rich membrane protrusions mediating ECM degradation; a soluble secreted form is also generated by cleavage of the GPI anchor.
• Expressed in the brain, notably in neurons of the rolandic area; also detected in various tissues at the protein level.
• Heavily glycosylated (predicted molecular weight ~37 kDa, observed as multiple bands 31–36 kDa due to differential glycosylation), contains disulfide bonds forming three Ly-6/uPAR domains, and is anchored via a C-terminal GPI moiety.
• Multiple transcript variants exist, potentially yielding isoforms with distinct extracellular regions (reflected by multiple observed protein weights).

Experimental Guidance and Technical Tips

• For flow cytometry, use live unfixed cells to preserve surface epitope integrity; if intracellular detection is required, fix/permeabilize with appropriate buffers.
• The immunogen (aa 23–303) covers the entire extracellular domain. Gentle cell harvesting (e.g., non-enzymatic dissociation) is recommended to avoid cleavage of the GPI anchor and loss of surface staining.
• Titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions, and include appropriate isotype controls.
• As a direct conjugate, no secondary antibody is needed. Consider using a viability dye for dead cell exclusion.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, connecting diagnostic developers and research laboratories with high-quality reagents tailored to their assay needs. This anti-CD87/PLAUR antibody supports studies in cancer invasion, inflammation, and tissue remodeling, and CamelBio can supply matched antibody pairs, recombinant uPAR proteins, and other ancillary materials to streamline your workflow. From rare-target monoclonal antibodies to bulk ancillary reagents, CamelBio helps bridge the gap between concept and clinical application.