Rabbit anti-Human CD87/PLAUR Monoclonal Antibody for Flow Cytometry - Q03405

Core Product Specifications and Parameters

Biological Background: CD87/PLAUR (Urokinase Plasminogen Activator Receptor) Function and Localization

• CD87, also known as urokinase plasminogen activator surface receptor (uPAR), is a GPI-anchored glycoprotein encoded by the PLAUR gene. Alternatively spliced isoforms and a secreted form exist.
• uPAR serves as a high-affinity receptor for urokinase-type plasminogen activator (uPA). Upon uPA binding, it activates plasminogen to plasmin, leading to extracellular matrix degradation and facilitating cell migration and tissue remodeling. Related references: PMID:1689240, PMID:15677461
• Beyond proteolysis, uPAR interacts with vitronectin, an extracellular matrix component, mediating cell-matrix adhesion and regulating integrin signaling, thereby coordinating dynamic changes in cell adhesion and motility. Related references: PMID:17548516, PMID:25168639, PMID:28849762
• uPAR is primarily localized to the cell membrane, particularly enriched at cell projections such as invadopodia, and is also found in a secreted form.
• The receptor is expressed in various tissues including the brain, with specific expression in neurons of the rolandic area.
• uPAR contains multiple disulfide bonds, is N-glycosylated, and is attached to the membrane via a GPI anchor. It is a member of the Ly-6/uPAR/alpha-neurotoxin superfamily, characterized by three-finger protein domains.

Experimental Guidance and Technical Tips

• The immunogen comprises the extracellular domain (amino acids 23–303) of human CD87/PLAUR, which includes the uPA-binding region. This design likely yields an antibody that recognizes the native conformation of uPAR on the cell surface.
• The conjugate provides direct detection for flow cytometry, simplifying the staining protocol and reducing potential cross-reactivity from secondary antibodies. Validate the staining pattern with appropriate isotype controls and cell lines expressing CD87.
• For flow cytometry, prepare single-cell suspensions and block Fc receptors as needed. If working with adherent cells, ensure gentle detachment to preserve surface antigen integrity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio equips diagnostic manufacturers and research labs with end-to-end access to high-quality raw materials for CD87/PLAUR-related studies. Beyond this validated rabbit monoclonal antibody for flow cytometry, our portfolio includes optimized antibody pairs, recombinant proteins, and bulk ancillary reagents to support your urokinase receptor pathway research. From target validation to scaled production, CamelBio is your reliable partner for IVD raw material sourcing.