Rabbit anti-Human CD85j/ILT2 Monoclonal Antibody for Flow Cytometry - Q8NHL6

Core Product Specifications and Parameters

Biological Background: LILRB1 Function and Localization

• LILRB1, also known as ILT2, LIR-1, MIR-7, and CD85j, is a member of the leukocyte immunoglobulin-like receptor (LILR) family, functioning as an inhibitory receptor on various immune cells.
• Acts as a broad-spectrum receptor for classical and non-classical MHC class I molecules (HLA-A, -B, -C, -G, and -F) and the viral homolog UL18, delivering inhibitory signals that attenuate immune cell activation (PubMed:16455647, PubMed:28636952). Related references: PMID:16455647 PMID:28636952
• Upon ligand engagement, LILRB1 inhibits NK cell and cytotoxic T cell-mediated lysis, suppresses FcεRI signaling and serotonin release in mast cells/basophils, and blocks FcγRI-mediated phosphorylation and calcium mobilization in monocytes/macrophages (PubMed:11907092, PubMed:9285411, PubMed:9842885). Related references: PMID:11907092 PMID:9285411 PMID:9842885
• Plays a pivotal role in immune tolerance at the maternal-fetal interface by interacting with HLA-G–β2M complexes, thereby inducing decidual NK cells to secrete growth factors that support fetal development (PubMed:16455647, PubMed:19304799, PubMed:29262349). Related references: PMID:16455647 PMID:19304799 PMID:29262349
• Additionally, LILRB1 engagement on B cells reprograms them toward an immune-suppressive phenotype, contributing to regulation of humoral responses (PubMed:24453251). Related references: PMID:24453251
• LILRB1 is expressed on B cells, monocytes, various dendritic cell subsets, and decidual macrophages and NK cells at the protein level.
• LILRB1 is primarily localized to the cell membrane and can also be shed in a soluble form; its extracellular region contains four immunoglobulin-like domains, a transmembrane helix, and an intracellular immunoreceptor tyrosine-based inhibitory motif (ITIM) that recruits phosphatases to mediate inhibitory signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 24–458) of human LILRB1, suggesting the antibody likely recognizes an epitope accessible on live cells, making it suitable for flow cytometry without permeabilization.
• When staining for FC, titrate the antibody to determine the optimal concentration for your cell type and experimental conditions.
• Use appropriate isotype controls and Fc receptor blocking to ensure specificity.
• Verify positive staining in LILRB1-expressing immune cell populations (e.g., monocytes, B cells) as a biological control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers by providing validated IVD antibodies like this anti-LILRB1 monoclonal antibody for flow cytometry. In addition, we offer optimized antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing services to streamline your assay development from concept to clinic. Our portfolio covers immune checkpoint and immunoregulatory targets, helping you accelerate immuno-oncology and immune monitoring research.