Rabbit anti-Human CD85j/ILT2 Monoclonal Antibody for FC - Q8NHL6

Core Product Specifications and Parameters

Biological Background: CD85j/ILT2 (LILRB1) Function and Localization

• LILRB1 (also designated CD85j, ILT-2, LIR1, MIR7) is a leukocyte immunoglobulin-like receptor that serves as an inhibitory receptor for a wide range of MHC class I alleles, including HLA-A, HLA-B, HLA-C, HLA-G and HLA-F. Related references: PMID:16455647, PMID:28636952
• Upon ligand engagement, LILRB1 transduces inhibitory signals that down-regulate immune cell activation, including inhibiting FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium. Related references: PMID:11907092, PMID:9285411, PMID:9842885
• It recognizes peptide-bound HLA-G in complex with β2-microglobulin, triggering the secretion of growth-promoting factors by decidual NK cells, which is important for maternal-fetal tolerance. Related references: PMID:16455647, PMID:19304799, PMID:29262349
• LILRB1 expression in B cells reprograms them toward an immune suppressive phenotype. Related references: PMID:24453251
• The protein is expressed primarily on B cells, monocytes, and dendritic cell subsets, as well as on decidual macrophages and NK cells. Related references: PMID:20448110, PMID:24453251, PMID:9285411, PMID:9842885, PMID:19304799, PMID:29262349
• LILRB1 localizes to the cell membrane and is also found in a secreted form, featuring immunoglobulin domains, disulfide bonds, and post-translational modifications including glycosylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular region (amino acids 24-458) of human CD85j/ILT2, encompassing the MHC-binding immunoglobulin-like domains. This design may favor the generation of antibodies capable of recognizing native epitopes suitable for flow cytometry applications.
• For flow cytometry, consider titrating the antibody to determine the optimal concentration on human peripheral blood mononuclear cells (PBMCs) or isolated leukocyte subsets. Verify specificity by including appropriate isotype controls and testing on LILRB1-overexpressing cell lines.
• Given the broad MHC class I binding by LILRB1, researchers should account for potential competition with endogenous ligands in certain assay setups and consider pre-blocking strategies if necessary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material supply, offering a streamlined path from concept to clinic. The Rabbit anti-Human CD85j/ILT2 mAb exemplifies our commitment to providing high-quality immunological reagents for immune checkpoint and tolerance research. Beyond this product, we deliver validated antibody pairs, optimized monoclonal/polyclonal antibodies, fluorescent conjugates, and bulk ancillary reagents to meet your assay development needs. For rare or specialized targets like LILRB1, our sourcing expertise ensures rapid access to reliable raw materials.