Rabbit anti-Human CD85j/ILT2 Monoclonal Antibody for FC - Q8NHL6

Core Product Specifications and Parameters

Biological Background: CD85j/ILT2 (LILRB1) Function and Localization

• LILRB1 (CD85j/ILT2) is a member of the leukocyte immunoglobulin-like receptor (LIR) family and functions as an inhibitory receptor on immune cells.
• It recognizes a broad spectrum of MHC class I molecules (HLA-A, -B, -C, -E, -F, -G) and the viral MHC class I homolog UL18, mediating immune tolerance and evasion.
  Related references: PMID:16455647, PMID:28636952
• Upon ligand binding, LILRB1 recruits SHP‑1 and SHP‑2 phosphatases to its ITIM motifs, inhibiting downstream signaling pathways such as FCER1A‑mediated serotonin release and FCGR1A‑mediated calcium mobilization.
  Related references: PMID:11907092, PMID:9285411, PMID:9842885
• In decidual NK cells, interaction with HLA‑G–beta‑2‑microglobulin complexes triggers secretion of growth‑promoting factors, contributing to fetal‑maternal immune tolerance.
  Related references: PMID:19304799, PMID:29262349
• The protein is expressed on the surface of B cells, monocytes, dendritic cell subsets, NK cells, and decidual macrophages.
• Alternative splicing produces multiple isoforms with predicted molecular weights ranging from ~49 kDa to 71 kDa, and the mature protein is glycosylated and phosphorylated.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and panel.
• Include proper isotype controls and Fc receptor blocking reagents to minimize non‑specific binding.
• Use a standard surface staining protocol; avoid permeabilization agents unless an intracellular epitope is confirmed.
• Validate staining in LILRB1‑positive immune cell populations such as human PBMCs, purified B cells, or monocytes.
• Since the immunogen covers the extracellular domain (aa 24‑458), the antibody likely targets an extracellular epitope; consider competitive binding assays if assessing ligand‑blocking activity.
• (ex/em ~590/617 nm) is a bright and photostable fluorophore; protect samples from prolonged light exposure and use appropriate optical filters.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibodies, antigens, and ancillary reagents for immuno‑oncology and immune profiling applications. This ‑conjugated anti‑CD85j/ILT2 antibody streamlines multicolor flow cytometry workflows. For custom antibody development, bulk supply, or technical consultation, our end‑to‑end solutions bridge your diagnostic development from concept to market.