Rabbit anti-Human CD85i/j Monoclonal Antibody for Flow Cytometry - O75019/Q8NHL6

Core Product Specifications and Parameters

Biological Background: CD85i/j Function and Localization

• LILRA1 (CD85i), also known as leukocyte immunoglobulin-like receptor 6 (LIR-6), is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which includes both activating and inhibitory receptors.
• It functions as a transmembrane receptor and may act as a receptor for class I MHC antigens, suggesting a role in modulating immune responses.
• The protein is expressed on the plasma membrane of immune cells, with tissue specificity data indicating its presence in monocytes and B-cells.
• LILRA1 contains characteristic immunoglobulin domains, disulfide bonds, and glycosylation sites, classifying it as a glycoprotein within the immunoglobulin superfamily.
• Structural features include a signal peptide and a transmembrane helix, which anchor the receptor to the membrane and facilitate signal transduction.
• Alternative splicing generates multiple isoforms, contributing to functional diversity and potential regulatory mechanisms.
• LILRB1 (CD85j), targeted by this antibody alongside CD85i, is a closely related inhibitory receptor in the same LIR family, sharing structural homology and overlapping expression patterns.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal staining concentration for your specific cell type and experimental conditions.
• As the immunogen covers the extracellular region (aa 17-461) of CD85i, the antibody likely binds to an accessible epitope on live, unfixed cells.
• Validate staining specificity using appropriate isotype controls and cell lines with known CD85i/j expression, such as monocytes or B-cell lines.
• Because the antibody may cross-react with LILRB1 (CD85j), confirm target identity using knockout or knockdown models if precise discrimination is required.
• Use standard flow cytometry buffers and protocols; include Fc receptor blocking to reduce nonspecific binding when working with Fc receptor-expressing cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies. For immune cell profiling and receptor research, our anti-CD85i/j antibody and related reagents support robust assay development. Our sourcing capabilities extend to bulk ancillary reagents and rare target raw materials, helping you accelerate progress from concept to clinic.