Rabbit anti-Human CD85i/j Monoclonal Antibody for Flow Cytometry - O75019 / Q8NHL6

Core Product Specifications and Parameters

Biological Background: CD85i (LILRA1) Function and Localization

• CD85i, also known as LILRA1 (leukocyte immunoglobulin-like receptor subfamily A member 1) or LIR-6, is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which belongs to the immunoglobulin superfamily.
• It may function as a receptor for class I MHC antigens, contributing to adaptive immunity regulation.
• The protein is a single-pass type I transmembrane glycoprotein containing extracellular immunoglobulin-like domains, disulfide bonds, and a signal peptide.
• LILRA1 is detected primarily on the membrane of monocytes and B-cells, consistent with its role in immune cell signaling.
• Alternative splicing generates multiple isoforms, as noted in the reference proteome (UniProtKB/Swiss-Prot O75019).
• Key structural features include an extracellular region with immunoglobulin-like repeats and a cytoplasmic tail, though the precise signaling motifs remain undefined.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to , enabling one-step staining for flow cytometry without the need for secondary detection reagents.
• For optimal surface staining of human leukocytes, consider titrating the antibody and including appropriate isotype controls to establish gates and assess non-specific binding.
• As CD85i is expressed on monocytes and B-cells, validate the antibody's performance in these cell types using your experimental system.
• The immunogen (extracellular domain of CD85i) suggests the antibody likely recognizes native, membrane-bound CD85i/j; confirm reactivity under your fixation/permeabilization conditions if intracellular epitopes are required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, research laboratories, and clinical facilities with a comprehensive range of IVD raw materials, including validated antibodies like this anti-CD85i/j monoclonal. Whether you need bulk quantities of this fluorophore-conjugated antibody for immunophenotyping, customized antibody pairs for assay development, or rare target sourcing for immune receptor research, our supply chain accelerates your workflow from concept to clinic.