Rabbit anti-Human CD85d/ILT4 Monoclonal Antibody for FC - Q8N423

Core Product Specifications and Parameters

Biological Background: LILRB2/CD85d/ILT4 Function and Localization

• Leukocyte immunoglobulin-like receptor subfamily B member 2 (LILRB2), also designated CD85d, ILT4, LIR2, and MIR10.
• Functions as a receptor for a broad spectrum of class I MHC antigens, including HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles. Related references: PMID:11169396 PMID:12853576 PMID:16455647
• Involved in immune tolerance; recognizes HLA-G in complex with B2M and nonamer self-peptide to trigger differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, maintaining maternal-fetal tolerance. Related references: PMID:16455647 PMID:20448110 PMID:27859042
• Competes with CD8A for binding to class I MHC antigens and inhibits FCGR1A-mediated phosphorylation and intracellular calcium mobilization. Related references: PMID:11875462 PMID:12853576 PMID:9548455
• Localizes to the cell membrane.
• Expressed in monocytes, dendritic cells, NK cells, and B cells; notably enriched in tolerogenic IL10-producing dendritic cells and myeloid-derived suppressor cells during pregnancy. Related references: PMID:20448110 PMID:27859042
• Contains immunoglobulin domains, undergoes glycosylation and phosphorylation, and is subject to alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 22–461), providing targeting capability for cell surface-expressed LILRB2, suitable for flow cytometry applications.
• For flow cytometry, consider performing antibody titration to determine the optimal working concentration and include appropriate isotype controls to assess nonspecific binding.
• Validate the antibody in the relevant human sample system (e.g., peripheral blood mononuclear cells, dendritic cell subsets) to confirm specificity and sensitivity.
• Note that is a bright and photostable fluorophore; use standard blue laser (488 nm) excitation and 530/30 nm bandpass filter detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing capabilities. This anti-LILRB2 (CD85d/ILT4) antibody is suitable for immunology and immune-oncology research applications. Whether you need ready-to-use conjugates or custom services, CamelBio supports your development pipeline from concept to clinic.