Rabbit anti-Human CD85d/ILT4 mAb for FC - Q8N423

Core Product Specifications and Parameters

Biological Background: CD85d/ILT4 Function and Localization

• Receptor for class I MHC antigens; recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles. Related references: PMID:11169396, PMID:12853576, PMID:16455647
• Functions as an inhibitory receptor involved in the down-regulation of immune responses and the development of tolerance.
• Recognizes HLA-G in complex with B2M, triggering differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, thereby maintaining maternal-fetal tolerance. Related references: PMID:16455647, PMID:20448110, PMID:27859042
• Competes with CD8A for MHC-I binding and inhibits FCGR1A-mediated phosphorylation and calcium mobilization. Related references: PMID:11875462, PMID:12853576, PMID:9548455
• Expressed on the cell membrane of monocytes, dendritic cells, B cells, and at lower levels on NK cells; enriched in tolerogenic IL-10-producing dendritic cells and myeloid-derived suppressor cells during pregnancy.
• Contains immunoglobulin domains, disulfide bonds, and undergoes alternative splicing, alternative initiation, and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 22-461), which may facilitate recognition of the native receptor conformation; consider this when developing assays for soluble or membrane-bound forms.
• This conjugate is designed for flow cytometry; for optimal resolution, titrate the antibody and use appropriate isotype control and viability dye in your panel.
• Validate staining in the relevant human immune cell populations known to express ILT4, such as peripheral blood monocytes, dendritic cells, and B cells.
• For intracellular or functional assays, verify antibody performance under the required fixation/permeabilization conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to a comprehensive portfolio of IVD raw materials, technical services, and consulting—supporting every stage from concept to clinic. With this anti-ILT4 monoclonal antibody, we address key research areas in immunology, immune tolerance, and maternal-fetal interface studies. Our supply capabilities extend to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing of rare-target raw materials to accelerate your diagnostic development.