Rabbit anti-Human CD85d/e/i/j Monoclonal Antibody for Flow Cytometry - Q8N423 / Q8N6C8 / O75019 / Q8NHL6

Core Product Specifications and Parameters

Biological Background: CD85d (LILRB2) Function and Localization

• LILRB2 (CD85d/ILT4/MIR10) is a member of the leukocyte immunoglobulin-like receptor (LILR) family, encoded by the LILRB2 gene (GeneID: 10288). Multiple isoforms arise through alternative splicing and alternative promoter usage.
• It functions as an inhibitory receptor for a broad spectrum of class I MHC molecules, including HLA-A, HLA-B, HLA-C, HLA-G, and HLA-F, thereby down-regulating immune responses and promoting peripheral tolerance. Related references: PMID:11169396 | PMID:12853576 | PMID:16455647
• LILRB2 competes with CD8A for MHC class I binding and inhibits FCGR1A-mediated cellular protein phosphorylation and calcium mobilization, reinforcing its immunosuppressive role. Related references: PMID:11875462 | PMID:9548455
• In the context of pregnancy, LILRB2 recognizes HLA-G in complex with beta-2-microglobulin, driving the differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, which is essential for maintaining maternal-fetal tolerance. Related references: PMID:16455647 | PMID:20448110 | PMID:27859042
• The LILRB family includes several closely related inhibitory receptors (LILRB1-5). This monoclonal antibody, raised against the extracellular domain of CD85d, exhibits pan-reactivity with CD85e (LILRB1), CD85i (LILRB4), and CD85j (LILRB5) due to sequence homology.
• Subcellular localization is primarily at the cell membrane, consistent with its role as a transmembrane receptor.
• Expression is most prominent in monocytes, with additional presence in tolerogenic IL-10-producing dendritic cells, myeloid-derived suppressor cells, and at lower levels in NK cells and B cells. Related references: PMID:20448110 | PMID:27859042
• The protein is a glycoprotein and phosphoprotein, featuring multiple immunoglobulin-like domains, disulfide bonds, a transmembrane helix, and a signal peptide, hallmarks of the immunoglobulin superfamily.

Experimental Guidance and Technical Tips

• The fluorophore (excitation ~650 nm, emission ~665 nm) is suitable for standard flow cytometry instruments equipped with a red laser; consider panel design to minimize spectral overlap.
• The immunogen corresponds to amino acids 22–461 of human CD85d, encompassing the extracellular region; this design may contribute to the antibody’s cross-reactivity with related LILRB family members. Users should verify specificity in their target cell populations.
• As an anti-human antibody, validate performance in relevant human samples (e.g., PBMCs, monocyte-derived dendritic cells). Include appropriate isotype controls and titrate the antibody to optimize signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized B2B provider of IVD raw materials and technical services, supporting diagnostic manufacturers from concept to clinic. This anti-human CD85d/e/i/j monoclonal antibody is part of a broad portfolio tailored for immune function research, including validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare target proteins. Whether you need optimized monoclonal/polyclonal antibodies or assistance in scaling up production, CamelBio offers reliable one-stop solutions to strengthen your assay development pipeline.