Rabbit anti-Human CD84 Monoclonal Antibody for IF/ICC and FC - Q9UIB8

Core Product Specifications and Parameters

Biological Background: CD84 Function and Localization

• CD84 (SLAMF5) is a member of the SLAM family of receptors, which modulate both innate and adaptive immune responses through homo- or heterotypic cell-cell interactions.
• It functions as a self-ligand receptor, and its signaling is regulated by the presence of small cytoplasmic adapter proteins SH2D1A/SAP and SH2D1B/EAT-2.
• Homophilic interactions of CD84 enhance interferon gamma (IFNG) secretion in lymphocytes and induce platelet stimulation via an SH2D1A-dependent pathway.
• CD84 mediates natural killer (NK) cell cytotoxicity, dependent on SH2D1A and SH2D1B (By similarity).
• It serves as a marker for hematopoietic progenitor cells. Related references: PMID:11564780, PMID:12115647, PMID:12928397
• In mast cells, CD84 negatively regulates high affinity immunoglobulin epsilon receptor signaling, independent of SH2D1A and SH2D1B, involving FES and PTPN6/SHP-1. Related references: PMID:22068234
• CD84 positively regulates macroautophagy in primary dendritic cells by stabilizing IRF8, inhibiting its TRIM21-mediated proteasomal degradation. Related references: PMID:29434592
• Subcellular localization: CD84 is a cell membrane protein expressed predominantly in hematopoietic tissues including lymph node, spleen, peripheral leukocytes, macrophages, B-cells, monocytes, platelets, thymocytes, T-cells, and dendritic cells.
• The protein undergoes alternative splicing, leading to multiple isoforms with predicted molecular weights around 24-38 kDa. It is a glycoprotein with disulfide bonds and phosphorylation sites.

Experimental Guidance and Technical Tips

• Use directly for flow cytometry (FC) or immunofluorescence/immunocytochemistry (IF/ICC) without a secondary antibody, as the antibody is pre-conjugated to .
• Titrate the antibody to determine the optimal concentration for your specific experimental setup; typical starting dilutions for FC are 1:50-1:200, but confirm empirically.
• For FC, include appropriate isotype control (rabbit IgG1 ) to assess non-specific binding.
• For IF/ICC, fixation with 4% paraformaldehyde is recommended; permeabilization may not be necessary for cell surface CD84, but test if intracellular staining is desired.
• Validate the antibody in human hematopoietic cell lines or primary immune cells known to express CD84 (e.g., Raji, THP-1, or PBMCs).
• As the antibody detects multiple isoforms, note that staining intensity may vary across cell types due to differential isoform expression.
• Avoid repeated freeze-thaw cycles; store at 4°C protected from light.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For immunology and hematology research, this CD84 antibody complements our portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing capabilities. We ensure seamless procurement, helping you accelerate project timelines with reliable supply chain support.