Rabbit anti-Human CD84 Monoclonal Antibody for FC - Q9UIB8

Core Product Specifications and Parameters

Biological Background: CD84 Function and Localization

• CD84 (SLAM family member 5, LY9B, SLAMF5) is a single-pass type I membrane protein and a self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family.
• SLAM receptors triggered by homo- or heterotypic cell-cell interactions modulate the activation and differentiation of a wide variety of immune cells, bridging innate and adaptive immunity.
• CD84 enhances proliferative responses of activated T-cells, promotes interferon-gamma secretion in lymphocytes, and induces platelet activation via SH2D1A-dependent pathways.
• CD84 may serve as a marker for hematopoietic progenitor cells. Related references: PMID:11564780, PMID:12115647, PMID:12928397
• CD84 is required for prolonged T-cell:B-cell contact, optimal T follicular helper function, and germinal center formation, contributing to B-cell tolerance maintenance.
• In mast cells, CD84 negatively regulates high affinity immunoglobulin epsilon receptor signaling independent of SH2D1A and SH2D1B, involving FES and PTPN6/SHP-1. Related reference: PMID:22068234
• In macrophages, CD84 enhances LPS-induced MAPK phosphorylation and NF-kappaB activation, modulating cytokine secretion via ITSM 2.
• CD84 positively regulates macroautophagy in primary dendritic cells by stabilizing IRF8 and inhibiting TRIM21-mediated proteasomal degradation. Related reference: PMID:29434592
• Predominantly expressed in hematopoietic tissues (lymph node, spleen, peripheral leukocytes) and immune cell subsets including macrophages, B-cells, monocytes, platelets, thymocytes, T-cells, dendritic cells, and mast cells, with high expression in memory T-cells.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions.
• Use an appropriate isotype control and ensure single-cell suspension to minimize nonspecific binding.
• Protect the fluorochrome-conjugated antibody from light during storage and handling; avoid repeated freeze-thaw cycles.
• Validate the antibody performance in your sample system, considering cell viability and potential Fc receptor blocking if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes one-stop access to IVD raw materials like this CD84-specific antibody. From validated antibody pairs to optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing, we streamline your supply chain. Whether you need consistent lot-to-lot performance for flow cytometry-based immunophenotyping or custom reagent development, our team supports your projects from concept to clinic.