Rabbit anti-Human CD80/B7-1 Monoclonal Antibody for Flow Cytometry - P33681

Core Product Specifications and Parameters

Biological Background: CD80 Function and Localization

• CD80 (T-lymphocyte activation antigen CD80), also known as B7-1, BB1, or CD28LG, is a costimulatory molecule belonging to the immunoglobulin superfamily critical for T-lymphocyte activation. Related references: PMID:38467718
• It serves as a ligand for CD28, providing a secondary signal that augments MHC/TCR-mediated T-cell priming; it also binds CTLA4, a decoy receptor that inhibits T-cell responses. Related references: PMID:12196291, PMID:10583602, PMID:11279502
• CD28 engagement by CD80 activates NF-kappa-B and MAPK signaling cascades, leading to production of interleukin-2 and other cytokines. Related references: PMID:10438913
• CD28 costimulation through CD80 enhances glucose metabolism and ATP synthesis in T-cells via the PI3K/Akt pathway. Related references: PMID:12121659
• CD80 modulates PD-L1/PD-1 interactions to limit excessive inhibitory signaling and maintain immune balance. Related references: PMID:36727298
• The protein localizes to the cell membrane of antigen-presenting cells, including B-cells, where it orchestrates humoral immunity by regulating germinal center responses.
• It also acts as a receptor for adenovirus subgroup B, facilitating viral entry during infection.
• CD80 undergoes alternative splicing and post-translational modifications including glycosylation, disulfide bond formation, palmitoylation, and phosphorylation.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal dilution for your specific cell type and experimental conditions.
• Include appropriate isotype controls and fluorescence-minus-one (FMO) controls to set gates and distinguish positive populations.
• The immunogen spans the extracellular domain; the antibody may recognize both native and recombinant CD80.
• Consider validating the antibody in the relevant sample system (e.g., human peripheral blood mononuclear cells) to confirm reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials and technical expertise, serving as a single sourcing bridge for diagnostic manufacturers and research labs. For immunology targets like CD80, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your development from concept to clinic.