Rabbit anti-Human CD80/B7-1 Monoclonal Antibody for Flow Cytometry - P33681

Core Product Specifications and Parameters

Biological Background: CD80 Function and Localization

• Key costimulatory molecule of the immunoglobulin superfamily essential for T-lymphocyte activation. Related references: PMID:38467718
• Engages CD28 receptor on naive T-cells to amplify TCR-mediated signals, triggering NF-κB and MAPK pathways and thereby promoting cytokine secretion. Related references: PMID:12196291, PMID:10438913
• Interacts with CTLA4, a high-affinity inhibitory receptor, to limit T-cell responses and maintain immune homeostasis. Related references: PMID:10583602, PMID:11279502
• Stimulates glucose metabolism and ATP synthesis in T-cells through PI3K/Akt signaling downstream of CD28 engagement. Related references: PMID:12121659
• Modulates PD-1/PD-L1 inhibitory axis; fine-tunes immune checkpoints by limiting excessive PD-L1 engagement. Related references: PMID:36727298
• Expressed on B-cells, CD80 plays a critical role in germinal center reactions, facilitating T–B cell interactions essential for humoral immunity.
• Serves as a cellular receptor for adenovirus subgroup B entry.
• Located on the cell membrane of antigen-presenting cells including dendritic cells, macrophages, and activated B-cells.

Experimental Guidance and Technical Tips

• The immunogen used (extracellular domain, aa 35-242) targets the regions critical for ligand binding; consider this antibody for applications analyzing receptor-ligand interactions.
• For flow cytometry, validate the optimal antibody dilution in the relevant cell system (e.g., stimulated PBMCs, antigen-presenting cells) to ensure specific staining.
• This antibody is recommended for FC; for other applications like WB or IHC, additional validation on the target protein’s stability and conformation is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. This anti-CD80 antibody supports research in T-cell biology and immuno-oncology, and CamelBio can fulfill your procurement needs for rare targets or diagnostic-grade materials.