Rabbit anti-Human CD80/B7-1 Monoclonal Antibody for FC - P33681

Core Product Specifications and Parameters

Biological Background: CD80 Function and Localization

• CD80, also known as B7-1, is a T-lymphocyte activation antigen and a member of the immunoglobulin superfamily, serving as a crucial costimulatory molecule for T-cell activation (PubMed:38467718). Related references: PMID:38467718
• It functions as the primary ligand for CD28, providing an essential secondary signal that augments MHC/TCR-mediated activation of naive T-cells (PubMed:12196291). Related references: PMID:12196291
• CD28 engagement by CD80 triggers intracellular signaling cascades, including NF-kappa-B and MAPK pathways, leading to the production of various cytokines (PubMed:10438913). Related references: PMID:10438913
• CD80 also interacts with CTLA4, acting as a negative regulator of T-cell responses by competing with CD28 and blocking T-cell priming (PubMed:10583602, PubMed:11279502). Related references: PMID:10583602, PMID:11279502
• The CD28/CD80 costimulatory pathway enhances T-cell metabolism by stimulating glucose uptake and ATP synthesis through the PI3K/Akt signaling axis (PubMed:12121659). Related references: PMID:12121659
• CD80 plays a role in regulating PD-L1/PD-1 interactions, limiting excessive inhibitory signaling and thus modulating immune checkpoint balance (PubMed:36727298). Related references: PMID:36727298
• As a receptor, CD80 facilitates adenovirus subgroup B entry into host cells (microbial infection).
• CD80 is predominantly expressed on the surface of professional antigen-presenting cells, with alternative splicing and post-translational modifications including glycosylation, disulfide bond formation, palmitoylation, and phosphorylation contributing to its functional regulation.

Experimental Guidance and Technical Tips

• This -conjugated monoclonal antibody is designed for direct detection of human CD80 by flow cytometry, eliminating the need for secondary staining steps.
• Consider using the antibody to assess CD80 expression levels on the surface of antigen-presenting cells (e.g., dendritic cells, B cells, monocytes) or activated T cells in human samples.
• Optimize staining conditions by titrating the antibody and including appropriate controls such as an isotype control and unstimulated cells to establish basal expression levels.
• Given the immunogen spans the extracellular domain (aa 35-242), the antibody is expected to recognize the native conformation of CD80 on live cells, making it suitable for extracellular staining protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw-material sourcing, providing convenient access to high-quality antibodies, antigens, and ancillary reagents for immune checkpoint and T-cell activation research, including this CD80 monoclonal antibody and related targets. Whether you require validated antibody pairs, bulk-scale monoclonal antibodies, or assistance in sourcing specialized raw materials for rare or emerging targets, CamelBio's integrated supply chain accelerates your diagnostic development from concept to clinic. Our commitment to reliable, one-stop solutions ensures you stay focused on innovation while we handle the complexities of material procurement.