Rabbit anti-Human CD80/B7-1 mAb - P33681

Core Product Specifications and Parameters

Biological Background: CD80/B7-1 Function and Localization

• CD80 (B7-1) is a costimulatory molecule of the immunoglobulin superfamily that plays a crucial role in T-lymphocyte activation by providing the primary auxiliary signal to naive T-cells through binding to the CD28 receptor, which is constitutively expressed on T-cell surfaces. Related references: PMID:38467718 PMID:12196291
• CD80/CD28 engagement activates downstream signaling pathways including NF-kappa-B and MAPK, leading to the production of cytokines essential for immune responses. Related references: PMID:10438913
• CD80 also functions as an inhibitor of T-cell activation by acting as a ligand for CTLA4, a decoy receptor that blocks CD28-mediated priming, providing a checkpoint mechanism to regulate immune responses. Related references: PMID:10583602 PMID:11279502
• CD28/CD80 costimulation stimulates glucose metabolism and ATP synthesis in T-cells by activating the PI3K/Akt signaling pathway, supporting T-cell energetic demands. Related references: PMID:12121659
• CD80 acts as a regulator of PD-L1/PD-1 interactions to limit excessive engagement of PD-L1 and its inhibitory role, contributing to immune homeostasis. Related references: PMID:36727298
• CD80 is expressed on the surface of antigen-presenting cells (APCs) and plays a critical role in B-cell/T-cell interactions during germinal center responses, essential for humoral immunity. (By similarity)
• The protein localizes to the cell membrane and undergoes post-translational modifications including glycosylation, disulfide bond formation, palmitoylation, and phosphorylation.
• (Microbial infection) CD80 serves as a receptor for adenovirus subgroup B, mediating viral entry.

Experimental Guidance and Technical Tips

• This antibody, generated against the extracellular domain (aa 35-242) of human CD80, is recommended for flow cytometry (FC) applications.
• For FC, optimize antibody titration using the relevant human cell line or primary cells expressing CD80; consider including Fc receptor blocking to reduce non-specific binding.
• Validate the antibody's specificity by using appropriate positive and negative controls (e.g., CD80-transfected cells or known CD80-expressing cell lines such as B-lymphoblastoid cells).
• The immunogen covers the mature extracellular region, which may recognize both the full-length and soluble isoforms; verify performance in your specific assay system.
• Cross-reactivity has been confirmed for human CD80; for other species, check sequence homology and test experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to provide a comprehensive selection of IVD raw materials, including validated monoclonal antibodies like this anti-CD80 clone, along with recombinant proteins, assay buffers, and ancillary reagents. Our procurement network supports the efficient sourcing of bulk quantities and custom configurations, simplifying your transition from research to clinical applications. With expertise in immune checkpoint and T-cell activation targets, we can also help source rare-target raw materials and development services to accelerate your IVD projects.