Rabbit anti-Human CD79b/Igβ Monoclonal Antibody for IF/ICC, FC - P40259

Core Product Specifications and Parameters

Biological Background: CD79b/Igβ Function and Localization

• CD79b (also known as Ig-beta, B29) is the beta chain of the B-cell antigen receptor complex, essential for B-cell development and function.
• Together with CD79a, it initiates signal transduction upon BCR engagement, leading to receptor internalization, trafficking to late endosomes, and antigen presentation.
• It enhances CD79a phosphorylation, likely by recruiting kinases or protecting CD79a from dephosphorylation.
• CD79b is a single-pass type I transmembrane protein localized at the cell membrane; its extracellular region contains an immunoglobulin-like domain.
• Expression is restricted to B-cells, from early pro-B to mature stages, but absent in terminally differentiated plasma cells.
• Post-translational modifications include glycosylation, disulfide bond formation, phosphorylation, and ubiquitination, which regulate its signaling activity.
• Alternative splicing generates multiple isoforms, including a secreted form; the canonical isoform has a molecular weight of ~26 kDa, while a smaller ~14 kDa fragment may correspond to the extracellular domain.
• The "Disease variant" keyword indicates that mutations in CD79B can lead to immune dysregulation or B-cell malignancies, underscoring its clinical significance.

Experimental Guidance and Technical Tips

• For IF/ICC, permeabilization may be required to detect intracellular epitopes, though CD79b is a surface receptor. The fluorophore is bright and photostable, suitable for multi-color imaging.
• For flow cytometry, titrate the antibody to determine optimal concentration for your specific cell type. Include proper isotype controls for gating.
• The recombinant protein immunogen suggests specificity to the extracellular domain; thus, the antibody likely recognizes the native surface-exposed epitope.
• Since CD79b is B-cell specific, validate staining using B-cell lines (e.g., Raji, Daudi) and negative controls (T-cell lines like Jurkat).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers, labs, and research institutes, offering a comprehensive portfolio that includes this CD79b/Igβ antibody. In addition to validated antibody pairs and optimized monoclonal/polyclonal antibodies for B-cell targets, we supply bulk ancillary reagents and source rare targets to support your immunoassay development from concept to clinic.